星点设计-效应面法优化川麦冬总黄酮提取工艺  

Optimization of extraction process of total flavonoids from ophiopogonis radix by central composite design-response surface methodology

作　　者：银玲[1] 张杰红[1] 彭月[1] 韦正[1] 王晓宇[1] 刘友平[1] 

机构地区：[1]成都中医药大学药学院 中药材标准化教育部重点实验室 中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都610075

出　　处：《中药与临床》2012年第4期20-23,共4页Pharmacy and Clinics of Chinese Materia Medica

基　　金：四川省教育厅科技支撑计划项目(NO.2011SZ0068)

摘　　要：目的:星点设计—效应面法优选川麦冬总黄酮的提取工艺。方法:以乙醇浓度、液料比、提取时间为自变量,以川麦冬总黄酮含量得率为因变量,通过对自变量各水平的二项式拟合,用效应面法选取最佳工艺,并进行预测分析。结果:确定的最佳工艺为85%乙醇、液料比36:1、温度80℃、提取3h,提取次数1次。提取预测值与验证值偏差为3.76%。二项式拟合复相关系数平方r=0.9610。结论:星点设计-效应面法优化川麦冬的总黄酮提取工艺,精密度高,方法简便,可预测性较好。Objective:To optimize the extraction process of total flavonoids from ophiopogonis radix by central composite design-response surface method.Method:Ethanol concentration,solvent ratio and extraction time were independent variables.Extraction rate of total flavonoids in ophiopogonis radix was dependent variable.No-linear mathematic models were used to estimate the relationship between the independent and the dependent variables.Response surface methodology was used to optimize extraction process.Predictions were carried out by comparing the observed and predicted values.Result:Optimal conditions of extraction process were as following:refluxed one time at 80℃ with 36 ratio of 85% ethanol for 3 hours.The bias between the observed and the predicted values of extraction quantity was 3.76%.Regression coefficient of binomial fitting complex model was as high as 0.9610.Conclusion:The optimum model is simple,precise and highly predictive.

关 键 词：川麦冬 总黄酮 星点设计-效应面法 提取工艺 

分 类 号：TQ46[化学工程—制药化工]