植物染色体微切割、微收集及微扩增技术应用  

Technique Application of Plant Chromosome Microdissection,Microcellection and Microcloning

作　　者：高居荣[1] 崔法[1] 封德顺[1] 李兴锋[1] 王洪刚[1] 

机构地区：[1]山东农业大学,农学院,国家小麦改良中心泰安分中心,作物生物学国家重点实验室,山东泰安271018

出　　处：《实验室研究与探索》2010年第7期11-13,26,共4页Research and Exploration In Laboratory

基　　金：国家自然科学基金资助项目(30571156)

摘　　要：为使染色体微切割、微收集、微扩增技术在植物精细遗传图谱及物理图谱构建中有效应用,利用SLuCUT全自动激光显微切割系统对普通小麦"中国春"和中间燕麦草染色体进行了微切割、微收集和微克隆研究。结果表明,SLuCUT-A全自动激光显微切割系统使植物染色体的微切割、微分离和微收集技术操作简单、快速准确、污染轻、样本不被降解等;DOP-PCR微量扩增条件适于普通小麦和中间燕麦草微量染色体的高效扩增,通过非变性聚丙烯酰胺凝胶电泳可以快速、准确地分析判断微扩增产物的来源。For high-efficient utilization of chromosome microdissection,microcollection and microcloning technology in the construction of plant high-resolution genetic map and physical map,chromosomes of both common wheat Chinese Spring and Elytrigia intermedium were used by SLuCUT-A fully automatic laser microdissection system.The results indicate that SLuCUT-A fully automated laser microdissection system makes the technology of chromosome microdissection,microcollection and microcloning of easy-operation,quick,precise,low-level pollution as well as low-degree degradation of samples.The condition of DOP-PCR microcloning is suitable for high-efficient cloning of micro chromosomes of both common wheat Chinese Spring and Elytrigia intermedium.PCR products were analysed in 6% non-denaturing polyacrylamide gels and the the source of microcloning product can be revealed quickly and precisely.

关 键 词：染色体 微切割 微收集 微克隆 

分 类 号：Q781[生物学—分子生物学]