冬瓜子中胡芦巴碱的含量测定  

Determination of trigonelline content in Semen benincasae

作　　者：董芳[1] 万丽[1] 吕芳[1] 王云红[1] 陈曙[1] 

机构地区：[1]成都中医药大学药学院,四川成都610075

出　　处：《中药与临床》2010年第2期20-22,共3页Pharmacy and Clinics of Chinese Materia Medica

摘　　要：目的:建立冬瓜子中胡芦巴碱的含量测定方法。方法:采用Diamonsil C18柱(250×4.6mm,5μm);流动相甲醇-水-0.05%十二烷基磺酸钠溶液(5:15:80);检测波长265nm,流动相流速1.0mL.min-1。结果:胡芦巴碱在25.2～201.6μg.mL-1呈良好线性关系(r=0.9998),该方法平均回收率为99.76%,RSD0.99%。结论:所建方法准确、简便、为科学地评价冬瓜子药材的质量提供了依据。Objective:To establish a method for the determination of trigonelline content in Semen benincasae.Method:The separation was performed on a Diamonsil C18(250×4.6mm,5μm)column with the mobile phase of methanol-H20-0.05% sodium dodecylsulfate(5:15:80).The detection wavelength was 265 nm,and the flow rate was 1.0 mL·min-1.Result:The calibration curve of trigonelline was linear in the range of 25.2 μg·mL-1～201.6 μg·mL-1(r=0.9998).The average recovery rate was 99.70% and the RSD was 0.99%.Conclusion:The method is accurate and suitable for the determination of trigonelline in Semen benincasae.The method can be adopted for quality control of Semen benincasae.

关 键 词：冬瓜子 胡芦巴碱 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]