凉山虫草的薄层色谱研究  

TLC study on Cordyceps liangshanensis

作　　者：陈璐[1] 万德光[1] 任艳[1] 谢婷[1] 雷艳[1] 国锦琳[1] 刘显福 

机构地区：[1]成都中医药大学中药材标准化教育部重点实验室,四川成都610075 [2]甘孜食品药品检定所,四川康定626000

出　　处：《中药与临床》2010年第2期25-26,共2页Pharmacy and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金资助项目(No.30801522);四川省中医药管理局资助项目(2008-11)

摘　　要：目的:建立一种快速、有效的凉山虫草成分鉴别方法,完善药材的质量标准。方法:采用薄层色谱法,以腺苷为对照品,以三氯甲烷-乙酸乙酯-异丙醇-水-浓氨水(5:2:6:0.6:0.13)为展开剂,对凉山虫草进行薄层分析。结果:10批凉山虫草样品薄层色谱中,在与对照品色谱相同位置上均显相同颜色的斑点,且色谱斑点清晰,无拖尾,分离度良好。结论:该方法简便,快速,结果准确度高,重现性好,药材用量少,是一种行之有效的凉山虫草鉴别方法。Objective:To establish an effective quality control method of Cordyceps liangshanensis to perfect the quality standard.Method:TLC method was used.Adenosine was used as the reference substance and the mixture of chloroform,ethyl acetate,isopropanol,H2O and NH3·H2O(5:2:6:0.6:0.13) as the developing solvent.Result:The spots in the chromatogram of the test solution corresponding to the position of adenosine had the same colour.Conclusion:The method is accurate and simple,and can be used for the quality control of Cordyceps liangshanensis.

关 键 词：凉山虫草 腺苷 薄层色谱法 

分 类 号：R284.1[医药卫生—中药学]