应用彗星试验检测乌头类生物碱致细胞DNA损伤作用  

Applying comet assay to determine DNA damage induced by aconitum alkaloid

作　　者：饶朝龙[1] 彭成[1] 

机构地区：[1]成都中医药大学药学院中药材标准化教育部重点实验室,四川成都610075

出　　处：《中药与临床》2010年第2期36-37,53,共3页Pharmacy and Clinics of Chinese Materia Medica

基　　金：国家重点基础研究发展计划(2006CB504705);国家自然科学基金重点项目(30230410);成都中医药大学科技发展基金(ZRYB200805)

摘　　要：目的:研究乌头类生物碱对细胞DNA的损伤作用,以期在分子水平进一步阐明乌头类生物碱的毒效作用特征及其分子机制。方法:以500、100、50和10μg.mL-1乌头碱、次乌头碱或新乌头碱分别染毒HepG2细胞1.5h,应用24孔板彗星试验检测细胞DNA损伤。结果:乌头类生物碱作用组细胞平均拖尾长度和拖尾细胞率与生物碱浓度存在剂量反应关系,且100和50μg.mL-1作用组细胞平均拖尾长度与阴性对照组之间差异具有统计学意义(P<0.05)。结论:乌头类生物碱具有细胞DNA损伤作用。Objective:To determine the DNA damage induced by Aconitum alkaloid for further studying on the molecular mechanism of aconitum's clinic effect and toxicity and its characteristic.Method:The hepatic cell strain of HepG2 was treated with aconitine,mesaconitine and hypaconitine for 1.5h,at concentration of 500,100,50 and 10 μg·mL-1 respectively,then determined with comet assay.Result:There was significant difference of tail length between the experimental group at concentration of 100μg·mL-1 or 50 μg·mL-1 and group of negative control(P<0.05).Dose-response relationship was also observed.Conclusion:Aconitine,mesaconitine and hypaconitine can cause DNA damage,which means they are genotoxic.

关 键 词：乌头类生物碱 彗星试验 DNA损伤 

分 类 号：R285.5[医药卫生—中药学]