芍药苷油水分配系数的测定及pH对其的影响  

Determination of n-octanol/water partition coefficients of paeoniflorin and the effect of pH on it

作　　者：李东芬[1] 尹蓉莉[1] 吕懿平[1] 赵明琴[1] 李杰[1] 

机构地区：[1]成都中医药大学药学院中药材标准化教育部重点实验室中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都610075

出　　处：《中药与临床》2011年第4期21-23,共3页Pharmacy and Clinics of Chinese Materia Medica

摘　　要：目的:通过处方前研究,考察芍药苷的溶解特性,测定芍药苷在不同pH值缓冲液中的油水分配系数,为芍药苷脂质体的处方设计提供参考。方法:用振摇法测定芍药苷的溶解度及正辛醇-水分配系数,比较pH值对油水分配系数的影响。结果:芍药苷在水、氯仿中的溶解度分别为50.57mgmL-1、68.34mgmL-1;在水、pH值分别为2.0、2.5、4.0、5.0、5.8、6.8、7.8的磷酸盐缓冲液中的正辛醇-水分配系数分别为-0.47、-0.61、-0.76、-0.71、-0.50、-0.78、-0.98、-0.58。结论:芍药苷易溶于水和氯仿;pH对芍药苷的油水分配系数有一定的影响,在pH5.0缓冲盐溶液中的溶解度及油水分配系数最大。Objective:To provide reference for the prescription of paeoniflorin liposome by investigating the solubility and the n-octanol/water partition coefficients of paeoniflorin and the effect of pH on it. Method:The solubility and n-octanol/water partition coefficients of paeoniflorin were measured by shake-flask method, and the effect of pH on n-octanol/water partition coefficients was investigated. Result:The solubility of paeoniflorin in water and chloroform were 50.57 mg﹒mL-1 and 68.34 mg﹒mL-1, respectively. The n-octanol/water partition coefficients were -0.47, -0.61, -0.76, -0.71, -0.50, -0.78, -0.98, -0.58 in water and phosphate buffers of pH 2.0, 2.5, 4.0, 5.0, 5.8, 6.8, 7.8, respectively. Conclusion:Paeoniflorin is easily soluble in water and chloroform. The n-octanol/water partition coefficients of paeoniflorin are influenced by pH. And the maximum values of solubility and n-octanol/water partition coefficient are found in phosphate buffer of pH5.0.

关 键 词：芍药苷 PH值 油水分配系数 溶解度 

分 类 号：R284.1[医药卫生—中药学]