RP-HPLC法测定山豆根中三叶豆紫檀苷的含量  

Determination of trifolirhizin in Radix et Rhizoma sophorae tonkinensis by RP-HPLC

作　　者：邓放[1] 吴兵[1] 董小萍[1] 

机构地区：[1]成都中医药大学药学院 中药材标准化教育部重点实验室 中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都610075

出　　处：《中药与临床》2012年第1期5-6,共2页Pharmacy and Clinics of Chinese Materia Medica

基　　金：成都中医药大学校基金课题(课题编号:ZRYB200415)

摘　　要：目的:建立RP-HPLC测定山豆根中三叶豆紫檀苷含量的方法。方法:色谱柱为Symmetry shield RP C18柱,流动相为甲醇-水(50:50),检测波长为310nm。结果:三叶豆紫檀苷在0.49～4.9μg范围内与峰面积有良好的线性关系;精密度试验相对标准偏差为1.31%;加样回收率为96.69%～98.57%;山豆根中三叶豆紫檀苷含量为0.019%。结论:本法操作简便,结果准确,可用于山豆根中三叶豆紫檀苷含量的测定。Objective: To develop a method to determine trifolirhizin in Radix et Rhizoma sophorae tonkinensis by RP-HPLC. Method: The analytical column was symmetry shield RP C18 column.The mobile phase was methanol-water(50:50). Result: The detection wavelength was set at 310 nm. The calibration curve was linear in the range of 0.49 μg～4.9μg for trifolirhizin. The relative standard deviation (RSD) of precision test was 1.31%.The recovery was between 96.69% and 98.57%. Trifolirhizin contents in Radix et Rhizoma sophorae tonkinensis was 0.019%. Conclusion: The method is simple, quick, accurate,and reliable for the determination of trifolirhizin in Radix et Rhizoma sophorae tonkinensis.

关 键 词：反相高效液相色谱 山豆根 三叶豆紫檀苷 

分 类 号：R286.0[医药卫生—中药学]