紫外—可见分光光度法测定细毡毛忍冬中总黄酮的含量  

Determination of the total flavonoids in Lonicera similes Hemsl. by UV-Vis spectrophotometry

作　　者：郑光雅[1] 马逾英[1] 翟萌[1] 穆向荣[1] 金丽鑫[1] 

机构地区：[1]成都中医药大学 中药材标准化教育部重点实验室,四川成都611137

出　　处：《中药与临床》2012年第2期18-20,共3页Pharmacy and Clinics of Chinese Materia Medica

基　　金：四川省教育厅重点项目课题川银花主流品种细毡毛忍冬特征性成分的分离及含量测定研究;项目编号:10ZA085专利号:201110344344.0

摘　　要：目的:建立一种简单、快速的方法,测定细毡毛忍冬中总黄酮含量,为细毡毛忍冬的质量控制和开发利用提供科学依据。方法:以芦丁为对照品,采用紫外分光光度法,在波长510nm处测定细毡毛忍冬总黄酮含量。结果:芦丁对照品溶液在8.0179～48.1074μg·mL-1浓度范围内,吸光度与浓度呈良好线性关系,细毡毛忍冬中总黄酮含量为3.38%。结论:该测定方法稳定、快速、简便,为细毡毛忍冬的质量控制和开发利用提供了科学依据。Objective: The study is aimed to establish a simple and quick method to determine the total flavonoids content in Lonicera similes Hemsl., to provide scientific evidence for the quality control and development of Lonicera similes Hemsl. Method: With rutin as the reference substance,the total flavonoids content was detected at 510 nm using UV spectrophotometry. Result: The linear relationship was good (r= 0.9999) in the range of 8.0179 μg·mL-1 ~ 48.1074 μg·mL-1 and the content of total flavonoids in the samples was 3.38%.Conclusion: UV speetrophotometry is a suitable method for the detection of the total flavonoids content in Lonicera similes Hemsl. for its simple and quick determination. And it will provide the scientific evidence for the quality control and development of Lonicera similes Hemsl.

关 键 词：细毡毛忍冬 紫外-可见分光光度法 总黄酮 芦丁 

分 类 号：R282[医药卫生—中药学]