紫花苜蓿(medicago sativa)抗旱相关促分裂原活化蛋白激酶MAPK基因的克隆  

Cloning of MAPK Gene Which was Activated by Drought in Medicago sativa

作　　者：白雪梅[1] 

机构地区：[1]内蒙古化工职业学院

出　　处：《内蒙古石油化工》2011年第5期18-20,共3页Inner Mongolia Petrochemical Industry

摘　　要：用异硫氰酸胍方法提取紫花苜蓿超级阿菠萝(Apollo Supreme)植株嫩叶和幼根的总RNA;采用RT-PCR的方法获得紫花苜蓿超级阿菠萝抗旱相关促分裂原活化蛋白激酶MAPK基因中MMK4基因的cDNA序列全长。cDNA序列测定表明:我们克隆到的MMK4基因的cDNA序列全长1116bp,由此推导的氨基酸序列与GeneBank上MMK4(X82270)基因的cDNA序列所编码的氨基酸序列完全相同,但其中有14个氨基酸的密码子有所不同。Total RNA of Medicago sativa(Apollo Supreme)'s seedling leaves and roots was isolated using the acid guanidium thiocynnate-phenol-chloroform extraction methed.A full cDNA sequence of MAPK(mitogen-activated protein kinase) was cloned by RT-PCR.Sequnce analysis showed clear similarity to MMK4(X82270) gene in GeneBank,and it's amino acid sequence identity to that encoded by MMK4(X82270) gene of Medicago sativa.But it's fourteen amino acid have their own codon.

关 键 词：紫花苜蓿 mapk级联 CDNA 抗旱相关 

分 类 号：S542.035.3[农业科学—作物学]