The effect of Hath1 over-expression in of guinea pig cochlea at one month after noise damage  

作　　者：GUO Wei-wei YANG Shi-ming 

机构地区：[1]Department of Otolaryngology,Head&Neck Surgery,Institute of Otolaryngology,Chinese PLA General Hospital,Beijing 100853,China

出　　处：《Journal of Otology》2010年第1期30-33,共4页中华耳科学杂志(英文版)

基　　金：supported by a Natural ScienceFoundation of China (NSFC) grant (# 30571017) to SMY;Joint Research Fund for Overseas Chinese Young Scholars by NSFC (# 30628030) to DH and SMY;NIH grant R01 DC 004696 from the NIDCD to DH

摘　　要：Objective To study effects of Adenovirus -mediated Hath1 expression in guinea pig cochlea at one month after exposure to intensive noise. Methods Normal hearing guinea pigs, weighing 250-300g, received exposure to 200 rounds of impulse noise at 170 dB sound pressure level (SPL). The virus vector was inoculated into the left cochlea 1 month after noise exposure. Animals were tested using ABR and prepared for morphological examinations includeing immunocytochemistry and SEM 4 weeks after vector inoculation. Results The adenovirus mediated report gene expressed in the damaged area. There were no significant differences between treated and control animal in ABR threshold and morphologic changes. No new hair cells appeared in the Hath1 treated animals. Conclusion Forced hath1 over-expression in the cochlea 1 month after noise exposure does not lead to appearance of new hair cells.Objective To study effects of Adenovirus -mediated Hath1 expression in guinea pig cochlea at one month after exposure to intensive noise. Methods Normal hearing guinea pigs, weighing 250-300g, received exposure to 200 rounds of impulse noise at 170 dB sound pressure level (SPL). The virus vector was inoculated into the left cochlea 1 month after noise exposure. Animals were tested using ABR and prepared for morphological examinations includeing immunocytochemistry and SEM 4 weeks after vector inoculation. Results The adenovirus mediated report gene expressed in the damaged area. There were no significant differences between treated and control animal in ABR threshold and morphologic changes. No new hair cells appeared in the Hath1 treated animals. Conclusion Forced hath1 over-expression in the cochlea 1 month after noise exposure does not lead to appearance of new hair cells.

关 键 词：hair cell damage Hath1 noise 

分 类 号：R764[医药卫生—耳鼻咽喉科]