Measurement of Ca^(2+) Flow in Cochlear Cells Using Non-Invasive Micro-Test Technique  

作　　者：CHEN Shi-qin YU Ning YE Sheng-nan YANG Shi-ming ZHAI Suo-qiang 

机构地区：[1]Department of Otolaryngology Head and Neck Surgery,Oto-Neurobiology Centre,Institute of Otolaryngology,Chinese PLA General Hospital,Beijing 100853,China [2]Department of Otolaryngology,Affiliated First Hospital,Fujian Medical University,Fuzhou 350005,China

出　　处：《Journal of Otology》2010年第2期90-96,共7页中华耳科学杂志(英文版)

基　　金：supported by NSFC grant(30600700,30772413)

摘　　要：Objective To test Calcium ion(Ca2+) flow at the head and end of outer hair cells(OHCs) in resting state and in response to Nimodipine treatment.Methods Non-invasive micro-test techniques were used to study Ca2+ in isolated OHCs in adult guinea pigs.Results Four types of Ca2+ transport were identified in OHCs on basilar membrane tissue fragments:influx at the head of with efflux at the bottom(type 1):efflux at the head of OHCs with influx at the bottom(type 2);influx at the both head and bottom(type 3);and efflux at the both head and bottom(type 4).However,only type 1 and type 3 of Ca2+ ion transport were detected in the cochlea.We propose that Ca2+ ion transport exists in adult guinea pig cochlear OHCs in resting state and is variable.Ca2 + flow in OHC can be inhibited by Nimodipine in resting state.Objective To test Calcium ion(Ca2+) flow at the head and end of outer hair cells(OHCs) in resting state and in response to Nimodipine treatment.Methods Non-invasive micro-test techniques were used to study Ca2+ in isolated OHCs in adult guinea pigs.Results Four types of Ca2+ transport were identified in OHCs on basilar membrane tissue fragments:influx at the head of with efflux at the bottom(type 1):efflux at the head of OHCs with influx at the bottom(type 2);influx at the both head and bottom(type 3);and efflux at the both head and bottom(type 4).However,only type 1 and type 3 of Ca2+ ion transport were detected in the cochlea.We propose that Ca2+ ion transport exists in adult guinea pig cochlear OHCs in resting state and is variable.Ca2 + flow in OHC can be inhibited by Nimodipine in resting state.

关 键 词：Guinea pig outer hair cells Ca2+ ion non-invasive micro-test technique nimodipine. 

分 类 号：R764[医药卫生—耳鼻咽喉科]