玉竹提取物A对免疫性肝损伤小鼠细胞因子的影响  

The therapeutic effects of EA-PAOA on the changes of cytokine in liver injury induced by Concanavalin A in mice

作　　者：赵良中[1] 陈爽[1] 李妍[1] 

机构地区：[1]吉林医药学院病原生物学教研室,吉林吉林132013

出　　处：《吉林医药学院学报》2009年第6期316-318,共3页Journal of Jilin Medical University

摘　　要：目的研究玉竹提取物A(EA-PAOA)对免疫性肝损伤小鼠细胞因子的影响,探讨其发挥抗肝损伤作用的可能机制。方法将昆明小鼠随机分成正常对照组、模型对照组、实验1组(EA-PAOA 0.5 g/kg)、实验2组(EA-PAOA 1 g/kg)、实验3组(EA-PAOA 2 g/kg)。除正常对照组外,其余各组均尾静脉注射20 mg/kg的刀豆蛋白A(ConA)0.5 mL,1次/d,连续5 d。每天注射ConA 2 h后,实验组分别腹腔注射不同浓度的EA-PAOA0.5 mL,3次/d,共4 d,第5天注射ConA后2 h注射1次EA-PAOA。末次注射EA-PAOA 6 h后摘眼球取血,测细胞因子。取肝脏组织进行病理学检查。结果3个实验组血清中IFN-γ和TNF-α水平明显降低,IL-10水平明显升高,并呈剂量依赖关系,与模型对照组相比差异显著。肝脏组织病理改变较模型对照组明显改善。结论EA-PAOA可能是通过降低血清中IFN-γ和TNF-α水平、升高IL-10水平以拮抗免疫性肝损伤作用。Objective To investigate the therapeutic effects of EA-PAOA on the changes of cytokine in immune hepatic injury in kunming mice and study its probable therapeutic mechanisms.Methods Kunming mice were divided into five groups:normal control,model control,test 1(EA-PAOA 0.5 g/kg),test 2(EA-PAOA 1 g/kg),test 3(EA-PAOA 2 g/kg).Model control and the three test groups were given intravenous injections of Concanavalin A(20 mg/kg)0.5 mL respectively,once one day,for five days in all.Two hours after the first injection of Concanavalin A,three test groups were given intraperitoneal injections of EA-PAOA 0.5 mL,three times one day,four days in all.On the fifth day,they were only given injection of EA-PAOA once.Six hours after the last injection of EA-PAOA,Blood were collected,the liver was reserved for routine light microscopy.Results The level of serum concentration of IFN-γ,TNF-α were lower,IL-10 were higher in the three EA-PAOA test groups compared to model control group,and showed dose independence.Significant difference was found compared to model control group(P<0.01).Conclusion The results indicated that EA-PAOA could obviously increase serum concentration of IL-10,and decrease serum concentration of IFN-γ,TNF-α,and played its anti-liver injury function.

关 键 词：免疫性肝损伤 刀豆蛋白A 细胞因子 玉竹提取物A 

分 类 号：R284.2[医药卫生—中药学]