微波萃取HPLC测定北冬虫夏草中腺苷的新方法研究  

Study on extraction method of adenosine from Cordyceps with microwave sample and analysis by HPLC

作　　者：卢钢[1] 孙军 孙立伟[1] 高晓旭[1] 

机构地区：[1]北华大学分析测试中心,吉林吉林132013 [2]吉林市产品质量检验院,吉林吉林132000

出　　处：《吉林医药学院学报》2010年第3期128-131,共4页Journal of Jilin Medical University

基　　金：科技部国家科技支撑计划(No:2007BAI38B02)

摘　　要：目的探讨北冬虫夏草腺苷的提取方法和检测技术。方法用微波萃取装置提取腺苷,考查水、甲醇、乙醇三种溶剂的提取效果,并进一步考察了固液比、萃取温度、萃取时间的影响,并对HPLC法测定提取液的腺苷含量的方法进行了方法实验研究。结果以水为提取溶剂,温度50℃,固液比为1∶20,提取时间15 min时提取效率0.233%。选用C18柱(4.6 mm×250,5μm),流动相:水∶甲醇=85∶15,流速0.8 mL/min,检测波长270 nm条件下,腺苷准确度符合方法学要求。结论采用微波萃取法提取北冬虫夏草中的腺苷,利用HPLC测定其含量的方法具有实用性并有推广应用价值。Objective To study extraction method and detection technology of adenosine from Cordyceps.Methods Adenosine was extracted by microwave sample to examine the effects of extraction of three different solvents:water,methanol and alcohol,and effects of solid—to—liquid ratio,extraction temperature and time,the extracted amount was examined by HPLC.Results The extraction efficiency could reach 0.233% when the solvent was water,temperature was 50℃,solid—to—liquid ratio was 1∶20,extraction time was 15 mins.Adenosine accuracy met the requirements of methodology under the following conditions:C18 column(4.6 mm×250,5 μm),mobile phase:water∶methanol=85∶15,flow rate 0.8 mL/min,detection wavelength:270 nm.Conclusion The method used to extract adenosine from Cordyceps and to determine the content by HPLC was worth spreading.

关 键 词：北冬虫夏草 微波萃取 腺苷 高压液相色谱 

分 类 号：R284[医药卫生—中药学]