高效液相色谱法测定颠胃酸口服液中橙皮酊含量  

The Content Determination of Orange Peel Tincture in Dianweisuan Oral Liquid by HPLC

作　　者：钟明哲 陶平德[2] 陈芳[2] 常明泉[2] 

机构地区：[1]郧县中医院,湖北郧县442500 [2]湖北医药学院附属太和医院药学部,湖北十堰442000

出　　处：《湖北医药学院学报》2012年第1期53-55,共3页Journal of Hubei University of Medicine

摘　　要：目的:建立高效液相色谱法测定颠胃酸口服液中橙皮酊含量的方法。方法:色谱柱:Diamonsil-C18柱(250mm×4.6 mm,5μm);流动相:甲醇-醋酸-水(35∶4∶61),流速1.0 mL.min-1,检测波长283 nm,进样量10μL,柱温30℃。结果:橙皮酊在4.0～96.0μg.mL-1范围内线性关系良好,(r=0.9998),回收率为98.4%,RSD=1.14%(n=6)。结论:所建方法便捷、重现性好,适用于颠胃酸口服液中橙皮酊的质量控制。Objective To establish a HPLC method for the content determination of orange peel tincture in Dianweisuan oral liquid.Methods The column was Diamonsil-C18 column(250 mm×4.6 mm,5 μm),the mobile phase was methanol-acetic acid-water(35∶4∶61) with a flow rate of 1.0 mL·min-1,the detective wavelength was set at 283 nm,the injection volume was 10 μL,column temperature was 30 ℃.Results The linear range of orange peel tincture was within 4.0 to 96.0 μg·mL-1,(r=0.9998).The recovery rate was 98.4%,RSD=1.14%(n=6).Conclusion The method was convenient,reproducible,and could be used for the content determination of orange peel tincture in Dianweisuan oral liquid.

关 键 词：颠胃酸口服液 橙皮酊 高效液相色谱法 含量 

分 类 号：R927.2[医药卫生—药学]