前列腺素E1对肾缺血再灌注损伤大鼠内皮的Rho/Rho激酶信号通路的影响  

Prostaglandin E1 Contributes to Endothelial Rho/Rho Kinase Signal Pathway of Renal Ischemia Reperfusion Injury Rats

作　　者：孟建中[1] 李丹丹[1] 国伟[1] 贾凤玉[1] 于颖[1] 吕苏一[1] 刘文渊[1] 王素霞[1] 葛彦明[1] 

机构地区：[1]济南军区总医院,济南250031

出　　处：《生物医学工程研究》2011年第4期234-238,共5页Journal Of Biomedical Engineering Research

摘　　要：探讨前列腺素E1对肾缺血再灌注损伤大鼠内皮细胞Rho/Rho激酶信号通路的影响。建立大鼠IRI模型,将健康Wistar大鼠75只随机平均分为假手术组、模型组、治疗组,每组各25只;并按术后观察时间分为6、12、24、48、72 h 5个不同时段亚组,每组各5只。检测各组血清肌酐(Scr)、血管内皮生长因子(Vas-cular endothelial growth factor,VEGF)在血清中的浓度及在肾组织的表达,检测肾组织髓过氧化物酶(My-eloperoxidase,MPO)活性、RhoA蛋白表达的变化。结果表明:(1)缺血60 min,大鼠血清Scr水平及肾小管坏死评分值均较基线值升高,并于再灌注48 h后达高峰,72 h后下降近基线值。(2)肾组织RhoA、VEGF表达及MPO活性于再灌注后6 h开始升高,12 h达高峰,24 h后开始下降,但均较假手术组明显增加(P<0.05)。(3)PGE1治疗组SCr、RhoA、VEGF表达及MPO活性均明显低于模型组(P<0.05)。RhoA、VEGF表达及MPO活性增加,反映了IRI内皮细胞的损伤程度;PGE1通过"阻断"(off swithch)Rho/Rho激酶信号通路,改善IRI内皮-免疫微环境,减轻肾组织损伤。To approach the prostaglandin E1(PGE1) contributes to endothelial Rho/Rho kinase signal pathway of renal ischemia reperfusion injury(IRI) rats.75 healthy Wistar rats were randomized into sham-operated control,IRI control and PGE1 treated groups with 25 each.Meanwhile,each group was stratified further according to the postoperative observation periods(6 h,12 h,24 h,48 h and 72 h with 5 each).To detect the level of serum creatinine(Scr) and the vascular endothelial growth factor(VEGF) expressed in nephridial tissue.Also diversity of myeloperoxidase(MPO) activity and RhoA protein expression in nephridial tissue was monitored.Results showed that(1)Both the Scr level and the renal tubular necrosis score in rats were advanced to the baseline at 60 min after ischemia.It peaked at 48 h after IRI and close to baseline at IRI 72 h.(2)Expression of RhoA,VEGF in nephridial tissue and activity of MPO were increased at IRI 6 h,peaked at 12 h and decreased at 24 h and were all significantly higher than that of the sham-operated control group(P<0.05).(3)Expression of SCr,RhoA,VEGF and activity of MPO in PGE1 treated group were obviously lower than that of IRI control group(P<0.05).The increase of RhoA,VEGF expression and MPO activity reflect to the degree of endotheliocyte injury.PGE1 can ameliorate the endothelium-immune microenvironment and alleviate nephridial tissue injury by off-swithching the Rho/Rho kinase signal pathway.

关 键 词：RHO激酶 缺血再灌注 急性肾损伤 信号 血管内皮生长因子 

分 类 号：R318[医药卫生—生物医学工程]