新生大鼠心肌组织Telocytes的组织形态学研究  

The Research on the Morphological Characteristic of Telocytes in New Born Rat Heart

作　　者：段翠密[1] 陈成[1] 冯兰兰[1] 王春兰[1] 袁振良[1] 牟永潮[1] 刘志强[1] 李俊杰[1] 周瑾[1] 

机构地区：[1]军事医学科学院基础医学研究所前沿交叉学科研究室,北京100850

出　　处：《生物医学工程研究》2012年第1期1-4,共4页Journal Of Biomedical Engineering Research

基　　金：国家自然科学基金重点资助项目(31030032)

摘　　要：本研究以新生大鼠心肌组织为研究对象,分别采用组织学、免疫组织化学及甲苯胺蓝染色方法,对新生大鼠心肌组织中telocytes形态特征和免疫标志进行观察与分析。其中,组织学染色与甲苯胺蓝染色结果显示,新生大鼠心肌组织中存在大量间质细胞,其中telocytes的分布多紧贴于心肌纤维束周围或纤维之间,细胞呈现长梭形、纺锤形及三角形等多种形态,胞质少、有一至多条长而细的细胞突起,胞核结构较致密、着色较深。免疫组化染色结果进一步证实新生大鼠心肌组织中其不但表达间质细胞的免疫标志vimentin,有些还表达c-kit/CD117和CD34两种与干细胞相关的表面标志物。本研究从组织形态学的角度阐明了telocytes的基本特征和分布,为进一步揭示其功能奠定了在体形态结构上的基础。This research based on new born rat heart,and introduced the methods of histology,immunohistochemistry staining and toluidin blue staining to investigate and analysis the morphological characteristic and immunological marker of telocytes in new born rat heart.The results of histology and toluidin blue staining demonstrated that a great quantity of interstitial cells exit in new born rat heart,among the total,cardiac telocytes distributed between cardiac muscle fibers or around the fiber bundles.The morphology of cardiac telocytes was various,including long spindle-shape,fusiformate or triangle.Cardiac telocytes had one or more thin and long prolongations,while the cytoplasm was few,and the nucleus was compact and deeply colored.Immunohistochemistry staining further confirmed that cardiac telocytes in new born rat heart expressed not only the interstitial cell marker,vimentin;some of them were also immuno-positive for c-kit/CD117 and CD34,which are stem cells related markers.This research clarifies the basic characteristics and distribution of cardiac telocytes,and establishes morphosis foundation for further exploring the function of cardiac telocytes.

关 键 词：TELOCYTES 心肌组织 间质细胞 CAJAL细胞 心脏干细胞 

分 类 号：R318[医药卫生—生物医学工程]