小半夏汤对水貂呕吐模型作用机制的研究  

Antiemetic Effect and Mechanism of Small Pinelliae Decoction on Mink Vomit Model

作　　者：任绪莹 牟群芳[1] 罗宜人[2] 王珏[1] 岳旺[1] 张芳[1] 

机构地区：[1]青岛大学医学院药理教研室 [2]青岛大学医学院附属医院胸外科

出　　处：《神经药理学报》2011年第3期23-26,共4页Acta Neuropharmacologica

基　　金：山东省自然科学基金项目(No.ZR2011HQ008);山东省中医药管理局项目(No.2007-158)

摘　　要：目的:研究小半夏汤(small pinelliae decoction,SPD)止呕作用及机制。方法:用顺铂(7.5 mg.kg-1)和阿扑吗啡(1.6 mg.kg-1)制备水貂呕吐模型。提取SPD(半夏生姜=1 1)有效成分制备成颗粒,给予水貂灌胃(ig)SPD(相当于给予4.0,1.0 g.kg-1生药量)30 min后,再腹腔注射(ip)顺铂或阿扑吗啡,研究SPD对外周和中枢性两种呕吐模型的抑制作用及机制。结果:SPD可显著抑制顺铂(72 h内)引起的急性和延迟性呕吐,减少干呕和呕吐次数并延长呕吐潜伏期(P<0.01);并可对抗顺铂引起极后区和回肠组织P物质I型受体(neurokinin-1,NK1)表达增加(P<0.05)。SPD也可抑制阿扑吗啡(6 h内)的呕吐反应(P<0.01);但对其呕吐潜伏期效果不显著,却可显著降低呕吐率(P<0.05)。结论:SPD颗粒对于顺铂和阿扑吗啡所导致的呕吐均有抑制作用,其作用机制与抑制中枢及外周的NK1受体表达有关。Objective:To study the antiemetic effect of small pinelliae decoction(SPD) and its mechanism.Methods:The active ingredients were extracted from SPD(pinellia tuber ginger=1 1)to make the granules.Cisplatin(7.5 mg.kg-1,ip)and apomorphine(1.6 mg.kg-1,ip) were used to prepare mink vomiting model.After 30 min of SPD(equivalent to 4.0,1.0 g.kg-1 crude drug)ig.adminstration,minks were administered of cisplatin or morphine to study the inhibitory effect and mechanism of SPD on the two models of vomiting.Results:SPD could inhibit acute and delayed vomiting induced by cisplatin(within 72 h)in the minks,reduce the number of retching and vomitin(gP<0.01),prolong the latency to vomit(P<0.01),and inhibit the expression of NK1 receptor in central(the area postrema)and peripheral(ileum tissue)tissues(P<0.05).SPD could also inhibit vomiting induced by apomorphine(within 6 h)(P<0.01),reduce significantly the ratio of vomiting(P<0.05)and did not affect the latency to vomit.Conclusion:SPD can inhibit vomiting induced by cisplatin and apomorphine,and its mechanism is related to the inhibition of central and peripheral NK 1 receptor expression.

关 键 词：小半夏汤 水貂 呕吐 顺铂 阿扑吗啡 

分 类 号：R28[医药卫生—中药学]