重组人睫状神经营养因子蛋白质定量检测方法的建立  

The establishment of the method for quantitative detection of rhCNTF protein

作　　者：马亚茹[1] 陆俭[1] 李萍[1] 高雪峰[1] 蒋琳[1] 

机构地区：[1]兰州生物制品研究所,兰州730046

出　　处：《微生物学免疫学进展》2011年第3期29-32,共4页Progress In Microbiology and Immunology

摘　　要：采用ELISA双抗体夹心法,建立一种快速灵敏的定量检测rhCNTF成品蛋白含量的方法。结果显示,rhC-NTF抗原浓度在(0～25)ng范围内线性良好(r>0.99),灵敏度为0.3ng/ml,与其他重组细胞因子无交叉反应,样品的检测结果与理论含量相吻合,CV<15%,该方法检测速度快、重复性好、灵敏度高、特异性好。A rapid and sensitive method for quantitative detection of rhCNTF protein was established by double antibody sandwich ELISA.The results showed that the linearity detection range of the rhCNTF concentration is 0-25 ng/ml(r>0.99),the sensitivity of the rhCNTF ELISA with a limit of detection is 0.3 ng/ml,it is of no cross reaction with other recombinant cytokines.The tested results of samples are tallied with the theoretical content data,CV(coefficient of variation) <15%.This method is of the advantage of rapid detection,the good reproducibility,highsensitivity and excellent specificity.

关 键 词：重组人睫状神经营养因子 双抗体夹心ELISA法 

分 类 号：R392-33[医药卫生—免疫学]