番茄中WRKY2基因的克隆  

Cloning of WRKY2 Gene in Tomato

作　　者：李娜[1] 于涌鲲[1] 赵福宽[1] 汤世坤[1] 孙清鹏[1] 于同泉[2] 路苹[2] 

机构地区：[1]北京农学院生物技术学院,北京102206 [2]农业应用新技术北京市重点实验室,北京102206

出　　处：《中国农学通报》2011年第2期95-98,共4页Chinese Agricultural Science Bulletin

基　　金：北京市自然科学基金"番茄LeWRKY基因功能分析"(5102015)

摘　　要：WRKY转录因子在植物应对生物或非生物胁迫的反应及生长和发育中起重要作用,为研究WRKY转录因子在番茄中的功能,本研究根据课题组已经克隆的番茄WRKY转录因子片段设计引物,采用RT-PCR方法,从JA诱导的番茄幼苗中,获得了608bp的cDNA片段。序列分析表明,该序列含有WRKYGQK保守结构域,与Capsicum annuum WRKY-c序列相似性是79%,与Nicotiana tabacum NtWRKY-7序列相似性是74%,表明已经成功克隆了番茄WRKY2基因片段。WRKY protein play an important role both in biotic and abiotic stress,also in growth and development of plant.In order to study the function of WRKY in tomato,the primers were designed according to the cloned WRKY transcription factors.The 608 bp fragment was obtained from tomato with RT-PCR method.Sequential analysis indicated that this sequence contained WRKYGQK conservative domain,and the similarity with Capsicum annuum WRKY-c and Nicotiana tabacum NtWRKY-7 were 79% and 74%,respectively.The results showed that WRKY gene sequence in tomato was cloned successfully.

关 键 词：番茄 WRKY转录因子 WRKY2基因 克隆 

分 类 号：S641[农业科学—蔬菜学]