黑龙江省瓜类白粉病菌IGS-RFLP分析  

IGS-RFLP Analysis of Powdery Mildew on Cucurbits in Heilongjiang Province

作　　者：魏尊苗[1] 王学征[1] 高鹏[1] 栾非时[1] 

机构地区：[1]东北农业大学园艺学院,黑龙江哈尔滨150030

出　　处：《中国蔬菜》2011年第20期28-33,共6页China Vegetables

基　　金：农业部"948"项目(2011-S17);国家西甜瓜产业技术体系分子育种岗位项目(CARS-26-02);黑龙江省自然科学基金重点项目(ZJNO705)

摘　　要：应用通用引物对采自黑龙江省不同地区的甜瓜、黄瓜、南瓜和西瓜等16份瓜类白粉病菌进行转录基因间隔区(IGS)分析,结果表明不同菌株的IGS区存在长度异质性和数量多态性。应用MspⅠ、AsuⅠ、Hinp1Ⅰ3个限制性内切酶对IGS扩增产物进行酶切(IGS-RFLP),不同菌株的酶切位点不同,电泳后产生多样性丰富的图谱,成为菌株特有的DNA指纹。利用NTSYS-PC软件对IGS1-RFLP酶切图谱进行数据分析,各菌株之间遗传相似系数的变化幅度为0.4600～0.8000,表明白粉病菌间有丰富的遗传多样性,以遗传相似系数0.60为阈值,供试菌株可区分为3个类群。初步确定葫芦科白粉病菌致病性与DNA多态性不形成对应关系,菌株的遗传多样性与菌株地理来源、寄主来源及设施类型亦无明显的直接关系。Intergenic spacer(IGS) was analyzed for 16 strains of powdery mildew using universal primers,which collected from melon,cucumber,pumpkin and watermelon on cucurbits in various regions of Heilongjiang Province.The results showed that there were length heterogeneity and polymorphism on IGS.IGS-RFLP digested by MspⅠ,AsuⅠand Hin p1Ⅰshowed that digest sites varied from the strains with profile polymorphism.The IGS-RFLP profile could become distinctive DNA fingerprint for strains.On the basis of IGS1-RFLP data analyzed by software NTSYS-PC,the similarity coefficient varied from 0.460 0 to 0.800 0,the isolates were clustered into 3 groups at the similarity coefficient of 0.60.No obvious association was found between DNA polymorphism and pathogen races differentiations as identified by biological tests on cucurbitaceae,also no association was found between the genetic diversity and any other of parameter tested:geographic,host origin or greenhouse type.

关 键 词：瓜类白粉病菌 基因间隔区(IGS) 限制性内切酶 

分 类 号：S436.42[农业科学—农业昆虫与害虫防治]