树舌多糖GF对HepA瘤细胞C-myc基因表达的影响  

The Effect of Ganoderma Appanatum Polysaccharides GF on C-myc Oncogene Expression of HepA Tumour

作　　者：宋高臣[1] 曾惜秋 于水澜[3] 于英君[3] 

机构地区：[1]牡丹江医学院生物化学与分子生物学教研室黑龙江省高校肿瘤疾病防治重点实验室,黑龙江牡丹江157011 [2]牡丹江市传染病医院,黑龙江牡丹江157011 [3]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中医药学报》2011年第4期36-38,共3页Acta Chinese Medicine and Pharmacology

基　　金：黑龙江省科技攻关项目(编号:G00C200902)

摘　　要：目的:探明树舌多糖GF对HepA瘤组织C-myc基因表达的影响。方法:应用免疫组化和原位杂交方法检测小鼠HepA瘤细胞中C-myc基因的mRNA与蛋白的表达情况。结果:与荷瘤组比较,树舌多糖组、猪苓多糖组中C-myc基因的mRNA与蛋白的表达量均明显降低(P﹤0.01),其中树舌多糖组与猪苓多糖组无显著性差异。结论:树舌多糖GF可通过降低C-myc mRNA量及C-myc蛋白的表达,而抑制HepA瘤细胞的增殖。Objective:To study the effect of GAPSGF on C-myc mRNA and N-ras protein expression in HepA tumour.Methods :Immunohistochemical staining and ISH were used to determine the effect of Ganoderma appanatum polysaccharides GF on C-myc mRNA and C-myc protein expression in mouse HepA tumor.Results:The average levels of C-myc mRNA and C-myc protein expression in GAPSGF group and PUPS group have much difference with control group.That is to say,GAPSGF and PUPS can significantly reduce the expression of C-myc mRNA and C-myc protein expression in HepA cells.Conclusion:Regulating the C-myc oncogene expression is one of the mechanisms of antitumor activity.

关 键 词：树舌多糖GF HEPA瘤细胞 C-MYC基因 

分 类 号：R28[医药卫生—中药学]