补肾生血法对慢性再生障碍性贫血患者信号转导MAPK通路的影响  

The Effect of Bushen Shengxue Granules on Signal Transduction Pathway MAPK of Patients with Chronic Aplastic Anemia

作　　者：施海涛[1] 王金环[2] 孙岸弢[3] 雍延礼[2] 孙伟正[2] 

机构地区：[1]黑龙江省大庆龙南医院肾内科,黑龙江大庆163000 [2]黑龙江中医药大学附属第一医院血液科,黑龙江哈尔滨150040 [3]中国中医科学院临床基础研究所,北京100000

出　　处：《中医药学报》2011年第4期49-52,共4页Acta Chinese Medicine and Pharmacology

基　　金：国家自然科学基金重大研究计划(编号:9070939);高等学校博士学科点专项科研基金(编号:20092327120001)

摘　　要：目的:观察补肾生血法对慢性再生障碍性贫血(CAA)的临床疗效,探索补髓生血颗粒对CAA患者骨髓基质细胞黏附信号转导MAPK通路的影响。方法:将CAA患者121例随机分为试验组60例、对照组61例进行临床试验研究,将患者治疗前后的骨髓基质细胞提取,分析黏附信号转导MAPK通路的相关酶类JNK、P38、ERK磷酸化的变化情况。结果:CAA患者骨髓基质细胞P-ERK1/2蛋白表达水平低于正常组(P<0.05),P-JNK与P-P38蛋白表达水平高于正常组(P<0.05);通过采用补肾生血法补髓生血颗粒治疗可以调整三种蛋白的异常表达,与治疗前比较(P均<0.05)。结论:CAA患者骨髓基质细胞造血粘附信号转导MAPK通路中酶类表达异常,这些可能是骨髓造血微环境粘附功能异常的原因之一,为CAA发病机制研究提供了分子生物学依据,本研究证明采用补肾生血法治疗CAA具有较好的疗效。Objective:To observe the clinical efficacy of Bushen Shengxue Granule(BSSXG) in chronic aplastic anemia(CAA) and discuss the differences in expression of signal transduction pathway MAPK before and after treatment.We observe the phosphorylation including ERK1/2,JNK,P38.Methods:124 cases of CAA were divided into two groups randomly.There are 61 cases who were used by BSSXG in experimental group,and 63 cases treatmented by Zaizaoshengxue Tablet in control group.The protein expression of enzymes P-ERK1/2,P-JNK,P-P38 of cultured bone marrow stromal cells were measured by Western blot.Results:1.The effects of BSSXG was superior to that of Zaizaoshengxue Tablet(P<0.05).2.The effect of Kidney-Yang deficiency type was superior to that of Kidney-Yin deficiency type(P<0.05).3.The expression level of P-ERK1/2 in CAA patients were lower than that in normal group(P<0.05).P-JNK and P-P38 protein levels were higher than that of normal group(P<0.05).Conclusion:BSSXG has a better clinical efficacy,and it is an effective drug for the treatment of CAA.

关 键 词：补肾生血法 慢性再生障碍性贫血 信号转导MAPK通路 

分 类 号：R25[医药卫生—中医内科学]