树舌多糖GF对小鼠肝癌H_(22)细胞P53及RB基因的影响  

The Effect of Ganoderma Appanatum Polysaccharides GF on P53 and RB Genes of Liver Cancer H_(22) Cells of Mice

作　　者：于英君[1] 王丽娜[1] 于水澜[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中医药学报》2011年第5期24-25,共2页Acta Chinese Medicine and Pharmacology

基　　金：中医药管理局科研计划项目(编号:5030131)

摘　　要：目的:为探明树舌多糖GF对肝癌H22瘤细胞RB基因及P53基因的影响。方法:用ELISA法检测RB基因及P53基因用药前后表达的差异。结果:树舌多糖GF组中P53基因、RB基因的表达量均显著高于荷瘤组(P<0.01)。树舌多糖组、环磷酰胺组无显著差异。结论:树舌多糖GF能使肝癌H22细胞P53及RB基因的表达上调。Objective:To study the effect on RB and P53 gene in H22 tumor cell with ganoderma appanatum polysaccharides GF(GAPSGF).Methods :Enxyme-linked immunoassay method was used to determine the RB and P53 gene expression level before and after treatment.Result: Significantly enhancing the RB and P53 gene expression compared with those in the control group(P<0.01).There was no significant difference between ganoderma appanatum polysaccharides GF and cyclophosphamide.Conclusion:Ganoderma appanatum polysaccharides GF can increase the expression of gene RB and P53 in hepatoma H22 tumor tissue.

关 键 词：树舌多糖GF H22肝癌细胞 P53基因 RB基因 

分 类 号：R28[医药卫生—中药学]