寒、热方剂对水浸应激型胃溃疡寒、热证模型大鼠胃组织SOD活性、MDA含量的影响  

The Influence of Cold or Heat Prescription on SOD Activity,MDA Contents in Gastric Tissue on Gastric Ulcer Induced by Bondage Water Immersion Stress Combined with Cold or Heat Syndrome Model Rats

作　　者：李冀[1] 毕珺辉[1] 柴剑波[1] 李胜志[1] 高彦宇[1] 肖洪彬[1] 赵雪莹[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中医药学报》2011年第5期57-59,共3页Acta Chinese Medicine and Pharmacology

基　　金："十一五"国家科技支撑计划项目-"方证相应"模式之复方药理研究(编号:2006BAI08B04-03);黑龙江省中医药管理局项目(编号:ZHY10-W01);哈尔滨市青年创新人才基金(编号:2009RFQXS003)

摘　　要：目的:探讨水浸应激型胃溃疡寒、热证模型的自由基代谢机制及寒、热方剂对该模型的自由基代谢的影响,阐明其方证相应的客观规律。方法:采用寒因素(冰水,0.3mol/LNaOH液)、热因素(8%干辣椒粉的60%乙醇混悬液)分别结合水浸应激法,建立实验性大鼠胃溃疡寒、热证模型。采用黄嘌呤氧化酶法(羟氨法)、硫代巴比妥酸法检测胃组织中SOD活力及MDA含量。结果:SOD方面,水应激胃溃疡组、水应激胃溃疡寒证组、水应激胃溃疡热证组与空白比较活力下降,有显著性差异(P<0.01),但寒证与热证间比较无明显差异。MDA方面,水应激胃溃疡组、水应激胃溃疡热证组、单纯热证组与与空白比较含量明显升高,有显著性差异(P<0.01),且以水应激胃溃疡热证组升高最为显著。大黄黄连泻心汤、理中丸可选择性针对相应的寒性、热性模型体现出对自由基代谢的调节作用。结论:通过"方证相应"理论验证了"病证结合"胃溃疡寒、热证模型的可行性。水浸应激型胃溃疡寒、热证模型存在自由基代谢异常。MDA可作为水浸应激型胃溃疡热证的微观指征。Objective:To explore the free radical metabolize mechanism of gastric ulcer model induced by bondage water immersion stress combined with cold or heat syndrome,and the influence of cold or heat prescription on free radical metabolizable mechanism.And to observe the basic mechanism of action and objective regularity of 'formula-syndrome corresponding' of cold or heat formula on gastric ulcer model combined with cold or heat syndrome.Methods: Adopting cold or heat factors combined with bondage water immersion stress methods to establish gastric ulcer model combined with cold and heat syndrome.Using xanthine oxidase method to detect the activity of SOD and thibabituric acid(TBA)method to detect the contents of MDA in gastric tissue.Results:Compared with the blank group,the activity of SOD decrease remarkably in gastric ulcer model induced by bondage water immersion stress group,gastric ulcer model induced by bondage water immersion stress combined with cold syndrome group、gastric ulcer model induced by bondage water immersion stress combined with heat syndrome group(P<0.01).But there was no difference between the cold syndrome group and the heat syndrome group.Compared with the blank group,the contents of MDA increase remarkably in gastric ulcer model induced by bondage water immersion stress group,gastric ulcer model induced by bondage water immersion stress combined with heat syndrome group、gastric heat syndrome group(P<0.01).Especially the gastric ulcer model induced by bondage water immersion stress combined with heat syndrome group.Dahuang Huanglian Xiexin decoction or Lizhong pill can respectively regulate the free radical metabolize of cold model or heat model.Conclusions:On the basis of the theory of 'formula-syndrome corresponding',verifying the feasibility of duplicating 'disease-syndrome combination' model,that is gastric ulcer model combined with cold or heat syndrome.There is the abnormal free radical metabolization in gastric ulcer model induced by bondage water immersion stress combined with cold

关 键 词：束缚水浸应激 胃溃疡 寒、热证 SOD MDA 

分 类 号：R28[医药卫生—中药学]