基于微电极阵列的多通道电生理检测系统的研制  

The Design and Fabrication of Microelectrode Array(MEA) and Multichannel Electrophysiology System

作　　者：韩尧[1] 汤戎昱[2] 周瑾[1] 林秋霞[1] 刘志强[1] 陈威震[1] 段翠密[1] 王春兰[1] 王常勇[1] 

机构地区：[1]军事医学科学院基础医学研究所前沿交叉学科研究室,北京100850 [2]中国科学院半导体研究所集成光电子学国家重点实验室,北京100083

出　　处：《生物医学工程研究》2012年第4期214-219,共6页Journal Of Biomedical Engineering Research

基　　金：国家自然科学基金资助项目(61178082)

摘　　要：设计并制备出一套低成本的微电极阵列及多通道电生理检测系统。通过基本的光刻、腐蚀对ITO导电玻璃进行加工,制备带有电极图形的导电玻璃,复合一层绝缘层后获得微电极阵列。利用软件设计PCB电路图并委托公司加工,获得相关系统的硬件部分。在labVIEW平台下进行编程,满足对数据采集卡的操作。我们成功制备了一套低成本的微电极阵列及相关检测系统。该系统用于不同类型神经细胞组成的神经网络自发放电检测,可在体外培养的神经网络中检测到5μV的神经信号,并在神经网络发育14 d检测到密集的同步簇发动作电位。我们研发的微电极阵列及多通道电生理检测系统具有良好的性能,可用于不同类型神经细胞的神经网络微弱信号的检测与神经网络发育研究。To design and fabricate a low cost microelectrode arrays and corresponding system.Microelectrode arrays was based on ITO glass chip,and was fabricated by photolithography,etching and insulating layer compositing.In this study,electric circuit was designed by software,PCB was fabricated by the commercial company,final packaging and debugging were finished in the lab.The corresponding software was made based on the labVIEW to manipulate the DAQ card.This system could be used to detect spontaneous activities of neural network based on different neurons.Results showed that the system could detect neural signals of 5 μV amplitude and the bursts on 14 DIV.The microelectrode arrays and system are performing well,and can be used to sense the electrical signals from different neuron-based neural network and investigate neural network development.

关 键 词：微电极阵列 神经信号 神经网络 动作电位 神经电生理 

分 类 号：R318.6[医药卫生—生物医学工程]