斯氏并殖吸虫抗原致大鼠脑组织病变的光镜、电镜观察  

Histopathological and ultrastructural observation of the brain from rat infected with Paragonimus skrjabini

作　　者：黄玉清[1] 李珍炼[2] 陈文碧[2] 佘俊萍[2] 杨朝鲜[3] 程基焱[3] 王光西[2] 

机构地区：[1]泸州医学院卫生科,泸州646000 [2]泸州医学院病原生物学教研室,泸州646000 [3]泸州医学院医学基础科研中心,泸州646000

出　　处：《中国人兽共患病学报》2012年第11期1107-1109,共3页Chinese Journal of Zoonoses

基　　金：四川省教育厅科研基金(No.2011538)资助~~

摘　　要：目的研究斯氏并殖吸虫大鼠脑病的病理改变。方法自并殖吸虫流行区采集溪蟹,分离斯氏并殖吸虫囊蚴,经腹腔注射感染犬,3个月后剖杀,检获斯氏狸殖吸虫成虫制备抗原,用于制作SD大鼠肺吸虫脑病模型。将6只SD雄性大鼠随机等分为并殖吸虫注射抗原的实验组、注射生理盐水的对照组。24h后解剖大鼠,取脑组织、固定、切片。光镜观察大鼠脑组织病理变化,电镜观察超微结构病理变化。结果 HE显示抗原注射大鼠脑血肿周围组织水肿明显,且局灶状坏死,神经元皱缩,尼氏小体溶解,周围出现间隙,神经突呈浅淡或消失,可见单核细胞及胶质细胞反应。电镜显示抗原注射组大鼠大脑皮质神经元胞体肿胀,核移位且形状不规则,核膜凸凹不平,核质溢出;染色质为絮状;线粒体肿胀,粗面内质网扩张呈囊泡化;脂褐素较多。结论肺吸虫抗原注射大鼠脑可引起脑组织光镜和超微结构改变:细胞肿胀,线粒体肿胀,内质网扩张。In order to study pathology change in the brain of experimental rat infected with paragonimiasis,six healthy adult of male SD rats were randomly divided into brain damage model group(n=3)and control group(n=3).Brain damage models were established by injecting adult worm antigen of Paragonimus skrjabini(P.skrjabini)into the brain with stereotactic apparatus of microinjection.Control group were injected by saline.Twenty-four hours after injected,rat brain tissue were collected,fixed and sliced.The histopathology and ultrastructure pathology change of the rat brain were studied by light and electron microscopy.There were brain tissue edema,focal necrosis,neuron shrinkage,dissolution of nissl,disappearance of neuritis,mononuclear cells and glial cells around the hematoma in the antigen injected rat under light microscopy.There were cerebral cortex neurons swelling,nuclear translocation and irregular shape,uneven nuclear membrane,nuclear transfer overflow,flocculent chromatin,mitochondrial swelling,expansion of rough endoplasmic reticulum,and more lipofuscin in the antigen injected rat under ultrastructural microscopy.The results suggest that there are histopathology and ultrastructure pathology change in brain of rats injected with antigen of P.skrjabini.

关 键 词：斯氏并殖吸虫 抗原 大鼠 脑组织 超微结构病理 

分 类 号：R383[医药卫生—医学寄生虫学]