基因芯片筛选畜禽热应激差异表达基因的研究进展  

Advances in Screening Differentially Expressed Genes Related to Heat Stress of Livestock and Poultry by Using cDNA Microarray

作　　者：刘丽莉[1,2] 谢红兵[1] 杨永生[1] 许丹宁[1] 李江长[1] 贺建华[1] 

机构地区：[1]湖南农业大学动物科学技术学院,长沙410128 [2]湖南科技大学生命科学学院,湘潭411201

出　　处：《动物营养学报》2012年第12期2287-2294,共8页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：湖南省高校科技创新团队支持计划资助;湖南省博士后基金项目(2011RS4006)

摘　　要：热应激可引起畜禽机体一系列基因水平的变化,直接影响各组织器官正常的生理功能。基因芯片技术已广泛应用于畜禽多种致病、应激等方面的基因表达谱研究。本文结合基因芯片技术在畜禽热应激上的研究和应用,对全面筛选畜禽器官氧化损伤、生产性能及体外细胞培养中与热应激相关的差异表达基因进行综述,以期为寻找和验证与畜禽热应激损伤密切相关的分子标记提供线索,并为修复畜禽热应激损伤的分子营养调控研究提供参考。A series of gene level changes in livestock and poultry caused by heat stress directly affect various organs and tissues of normal physiological function.The technology of cDNA microarray has been widely applied in pathogenic,stress and other aspects of the study of gene expression profile in livestock and poultry.The latest research and application advances in screening differentially expressed genes related to heat stress of livestock and poultry by using the cDNA microarray were summarized,including the analysis of the differentially expressed genes related to heat stress on oxidative damage of tissues and organs,performance,and in vitro cultured cells from different livestock and poultry.The application of cDNA microarray could provide some important methods for searching and validating the biological molecular markers closely related to the body damage caused by heat stress,and play an important reference role in the research of nutritional regulation for heat stress-induced injuries in livestock and poultry.

关 键 词：基因芯片 畜禽 热应激 差异基因 

分 类 号：S811[农业科学—畜牧学]