绝食法与无氮饲粮法测定黄羽肉鸡内源性氨基酸排泄量及变异的比较研究  

A Comparative Study of Fasting Method and Nitrogen-Free Diet Method for Determining Endougenous Amino Acid Excretion in Yellow-Feathered Chickens

作　　者：任立芹[1] 赵峰[1] 谭会泽 张建智[1] 米宝民[1] 赵江涛 张宏福[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室,北京100193 [2]广东温氏集团食品有限公司,新兴527439

出　　处：《动物营养学报》2012年第12期2424-2435,共9页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金项目(30901037);北京市科技新星计划项目(2011098);科技部创新方法工作专项(2009IM033100)

摘　　要：本试验旨在通过比较黄羽肉鸡在绝食和无氮饲粮条件下内源性氨基酸排泄量及其变异的差别,为鸡饲料可利用氨基酸评定中内源性氨基酸的测定提供参考。采用单因素完全随机设计,选择18周龄健康、体重基本一致的黄羽肉鸡72只,随机分成3组,每组6个重复,每个重复4只鸡。分4个批次重复测定绝食、强饲25 g无氮饲粮、强饲40 g无氮饲粮时内源性氨基酸排泄量。结果表明:绝食组、25 g无氮饲粮组、40 g无氮饲粮组内源性氨基酸排泄量依次显著增加(P<0.05)。在内源性氨基酸排泄量的变异上,绝食条件下,4个测定批次间17种内源性氨基酸排泄量的总变异系数在9.74%～19.99%,其中批内变异系数在9.97%～16.88%,批间变异系数在2.58%～16.23%,4个测定批次间内源性苏氨酸、胱氨酸、丝氨酸、脯氨酸排泄量有显著性差异(P<0.05)。强饲25 g无氮饲粮条件下,4个测定批次间17种内源性氨基酸排泄量的总变异系数在17.77%～31.33%,其中批内变异系数在14.99%～28.80%,批间变异系数在3.89%～17.04%,4个测试批次间内源性精氨酸、甘氨酸、脯氨酸排泄量差异显著(P<0.05)。强饲40 g无氮饲粮条件下,4个测定批次间17种内源性氨基酸排泄量的总变异系数在16.23%～33.39%,其中批内变异系数在17.15%～33.82%,批间变异系数在4.19%～16.29%,4个测试批次间内源性异亮氨酸和苯丙氨酸排泄量差异显著(P<0.05)。由此可见,内源性氨基酸排泄量随强饲量的增加而增加,强饲25与40 g无氮饲粮条件下的内源性氨基酸排泄量的变异系数非常接近,且都大于绝食条件下内源性氨基酸排泄量的变异。This experiment was conducted to compare the excretion and variation of endogenous amino acids(EAA) in yellow-feathered chickens under fasting and nitrogen-free diet(NFD) to evaluate the methods for the EAA determination in the measurement of the digestible amino acids of ingredients.Using a single factor completely randomized design,a total of 72 adult roosters aged 15 weeks were randomly selected according to their initial body weight and divided into 3 groups with 6 replicates per group and 4 roosters per replicate.The same experiment was repeated 4 batches to determine the EAA excretion under fasting,25 g NFD,and 40 g NFD,respectively.The results showed as follows: the EAA excretion was increased significantly from the groups of fasting,25 g NFD to 40 g NFD(P<0.05).In the fasting group,the total coefficient of variation(CVtotal),intragroup coefficient of variation(CVwithin),and intergroup coefficient of variation(CVbetween) of the EAA excretion of 17 amino acids among among 4 batches were from 9.74% to 19.99%,from 9.97% to 16.88%,and from 2.58% to 16.23%,respectively.The differences of EAA excretion of threonine,cystine,serine and proline among 4 batches were significant(P<0.05).In the 25 g NFD group,the CVtotal,CVwithin,and CVbetween of the EAA excretion of 17 amino acids among 4 batches were from 17.77% to 31.33%,from 14.99% to 28.80%,and from 3.89% to 17.04%,respectively.The differences of EAA excretion of arginine,glycine and proline among 4 batches were significant(P<0.05).In the 40 g NFD group,the CVtotal,CVwithin,and CVbetween of the EAA excretion of 17 amino acids among 4 batches were from 16.23% to 33.39%,from 17.15% to 33.82%,and from 4.19% to 16.29%,respectively.The differences of EAA excretion of isoleucine and phenylalanine among 4 batches were significant(P<0.05).It can be concluded that the excretion of EAA increases with the increasing amount of force-fed diet.The variation coefficients of the 25 and 40 g force-fed group are similar,and are both larger than those of fasting group.

关 键 词：内源性氨基酸 绝食法 无氮饲粮法 黄羽肉鸡 

分 类 号：S831[农业科学—畜牧学]