乙酸铜对小鼠睾丸细胞损伤的微核试验  

Detect the Damage of Copper Acetic Acid on Mouse Testis Cells by Micronucleus Test

作　　者：熊建利[1] 朱文文[1] 孙平[1] 张纪亮[1] 李猛[1] 

机构地区：[1]河南科技大学动物科技学院,河南洛阳471003

出　　处：《湖北农业科学》2012年第22期5134-5136,5141,共4页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(30900138);河南科技大学博士科研启动基金项目(09001367)

摘　　要：以昆明小鼠为试验材料利用微核试验方法研究了乙酸铜对昆明小鼠睾丸细胞的损伤。将78只昆明小鼠随机分为6组,每组13只,分别按每千克体重注射不同剂量的(0、0.5、1.0、2.0、4.0、8.0 mg)的乙酸铜,观察不同染毒时间(48、96、144、192、240 h)下小鼠睾丸细胞的微核率。结果表明,乙酸铜对小鼠睾丸细胞具有明显的毒性效应,能诱导微核的产生。各试验组中,微核率与乙酸铜浓度和染毒时间有关,微核率随乙酸铜浓度的增加和染毒时间的延长呈明显的上升趋势,并在最长的染毒时间(240 h)时的最高剂量组(每千克体重注射8.0 mg)达到最大值(8.56‰),即具有明显的剂量和时间效应。To research the reproductive toxicity of copper acetic acid on KM mouse,78 mice were randomly divided into six groups,and injected with different concentrations of copper acetic acid solution(0,0.5,1.0,2.0,4.0,8.0 mg/kg.w).And then the micronucleus rates were examined after different times(48,96,144,192,240 h).The results showed that the copper acetic acid had obvious toxic effect on mouse testis cells.It could induce the formation of micronucleus.The micronucleus rate in all experimental groups was correlated with the concentration of copper acetic acid and exposure time.The micronucleus rate had obvious increasing trend with increasing of copper acetic acid concentration and exposure time,and reached the maximum value(8.56‰) at the concentration of 8.0 mg/kg.w and exposure time of 240 h.It was indicated that the micronucleus rate induced by copper acetic acid had obvious concentration and time effects.

关 键 词：微核 微核试验 生殖毒性 乙酸铜 

分 类 号：Q343[生物学—遗传学]