L-茶氨酸对黄羽肉鸡生产性能和免疫功能的影响  

Effects of L-Theanine on Performance and Immune Function of Yellow-Feathered Broilers

作　　者：文慧[1] 魏时来[1] 张石蕊[2] 侯德兴[2] 肖文军[3] 贺喜[2] 

机构地区：[1]甘肃农业大学动物科技学院,兰州730070 [2]湖南农业大学饲料安全与高效利用教育部工程研究中心,长沙410128 [3]湖南农业大学国家植物功能成分利用工程技术研究中心,长沙410128

出　　处：《动物营养学报》2012年第10期1946-1954,共9页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金项目(30901035);湖南省自然科学基金项目(09JJ4015);教育部重点科研项目(211122);湖南省“百人计划”创新人才项目(2010);湖南省“畜禽应激生理与营养调控”高校科技创新团队项目(2010)

摘　　要：本试验旨在探讨L-茶氨酸对黄羽肉鸡生产性能和免疫功能的影响。选用1日龄黄羽肉鸡690只,随机分为6个处理,每个处理5个重复,每个重复23只。6个处理分别为对照组(饲喂无抗生素基础饲粮)、抗生素组[饲喂在基础饲粮中添加10%硫酸黏杆菌素的饲粮,前期(1～3周)添加200 mg/kg,后期(4～7周)添加150 mg/kg]、4个不同梯度(100、200、400和800mg/kg)L-茶氨酸试验组,试验期为49 d。试验检测了黄羽肉鸡的生产性能、免疫器官指数、肠道分泌性免疫球蛋白A(sIgA)含量及血清溶菌酶活性、抗体水平和免疫细胞因子含量等指标。结果表明:在试验前期,100 mg/kg L-茶氨酸组黄羽肉鸡的体增重显著低于其他各组(P<0.05),100、200和800 mg/kg L-茶氨酸组黄羽肉鸡的法氏囊指数显著低于对照组(P<0.05),各L-茶氨酸组黄羽肉鸡的肠道sIgA含量和血清干扰素-γ(IFN-γ)含量显著高于对照组(P<0.05)。在试验后期,与对照组及抗生素组比较,L-茶氨酸对黄羽肉鸡生产性能、免疫器官指数、肠道sIgA的含量影响不显著(P>0.05);100、200和400 mg/kg L-茶氨酸组黄羽肉鸡血清白细胞介素-2(IL-2)的含量显著高于对照组(P<0.05),200和400 mg/kg L-茶氨酸组黄羽肉鸡血清IFN-γ的含量显著高于对照组(P<0.05)。在整个试验期,各组黄羽肉鸡血清溶菌酶的活性和白细胞介素-4的含量差异不显著(P>0.05);一免后第6天,100和200 mg/kg L-茶氨酸组黄羽肉鸡血清新城疫抗体滴度显著低于对照组和抗生素组(P<0.05),一免后第14天,100、200和400 mg/kg L-茶氨酸组黄羽肉鸡抗牛血清白蛋白(BSA)抗体水平显著高于对照组(P<0.05),其他各阶段各组间的抗BSA抗体水平和新城疫抗体滴度均差异不显著(P>0.05)。由此可知,饲粮添加L-茶氨酸对黄羽肉鸡生长后期生产性能、免疫器官指数均无显著影响,说明其对黄羽肉鸡后期生长是安全的;同时L-茶氨酸可提高黄羽肉鸡生长前期肠道sIgA�The purpose of this study was to investigate the effects of L-theanine on the performance and immune function of yellow-feathered broilers.A total of 690 one-day-old yellow-feathered broilers were randomly divided into 6 groups with 5 replicates per group and 23 birds per replicate.The groups were divided into a control group(non-antibiotic group),an antibiotic group(added 10% of colistin sulphate,200 mg/kg during 1 to 3 weeks,150 mg/kg during 4 to 7 weeks),4 different levels of L-theanine(100,200,400 and 800 mg/kg) groups.The trial lasted for 49 days.Performance,immune organ indices,intestinal secretory immunoglobulin A(sIgA),serum lysozyme activity,antibody level and immunocytokine contents were investigated.The results showed as follows: 1) during the period of 1 to 3 weeks,the body weight gain of 100 mg/kg L-theanine group was significantly lower than that of the other groups(P<0.05),the bursa of fabricius index of 100,200 and 800 mg/kg L-theanine groups was significantly lower than that of the control group(P<0.05).The contents of intestinal sIgA and serum interferon-γ(IFN-γ) in L-theanine groups were significantly higher than those of the control group(P<0.05).2) During the period of 4 to 7 weeks,there were no significant differences in the performance,immune organ indices and intestinal sIgA content among L-theanine groups,control group and antibiotic group.Serum interleukin-2(IL-2) content of 100,200 and 400 mg/kg L-theanine groups was significantly higher than that of the control group(P<0.05),and serum IFN-γ content of 200 and 400 mg/kg L-theanine groups was significantly higher than that of the control group(P<0.05).3) During the entire experimental period,there were no significant differences in serum lysozyme activity and interleukin-4(IL-4) content among all groups(P>0.05).Lower anti-Newcastle disease virus(NDV) titers of 100 and 200 mg/kg L-theanine groups were found on day 6 post primary immunization compared with the control group and antibiotic group(P<0.05),but higher anti-bovine serum album

关 键 词：L-茶氨酸 黄羽肉鸡 生产性能 免疫功能 

分 类 号：S816.7[农业科学—饲料科学]