有毒甲藻Coolia monotis细胞密度测定方法的比较研究  

Comparative Study on Cell Counting Method with Toxic Dinoflagellate Coolia Monotis

作　　者：曾玲[1] 文菁[2] 梁计林[3] 龙超[3] 

机构地区：[1]湛江师范学院化学科学与技术学院,广东湛江524048 [2]湛江师范学院生命科学与技术学院,广东湛江524048 [3]中国科学院南海海洋研究所广东省海洋药物重点实验室,广东广州510301

出　　处：《湛江师范学院学报》2012年第3期86-90,共5页Journal of Zhanjiang Normal College

基　　金：湛江师范学院青年科研基金项目(Q11008);广东省自然科学基金资助项目(S2011040000463);广东省高校优秀青年创新人才培养计划项目(LYM11086);中国科学院海洋生物资源可持续利用重点实验室开放基金课题(LMB111004);湛江市科技攻关计划项目(2011C3104009)

摘　　要：采用显微镜计数法、荧光分光光度法、叶绿素a含量测定法、可见分光光度法对利玛Coolia monotis指数生长期不同浓度的培养液进行细胞密度测定.结果表明后3种细胞计数方法与显微镜计数法相比较,均有较好的线性关系;同时对不同生长期的Coolia monotis培养液进行测定的结果表明,叶绿素a含量测定法与显微镜计数法得到的生长曲线一致性最好,最能真实反映Coolia monotis的生长状态,其次是分光光度法,最差的是荧光测定法.比较分析发现,叶绿素a含量测定法适用于不同细胞密度范围的测定,具有简便、计数准确、适于批量操作的优点,可确定为便捷、有效、可行的Coolia monotis细胞计数法.In this study four cell-counting methods of Microscope,Fluorescence spectrophotography,Visible spectrophotography and Chla containing measurement were used to measure the amount of the exponential phase cell of Coolia monotis.The insightful comparison on the interrelations of the last three mentioned cell counting methods was made with the method of Microscope,the results revealed the sound 1inear interrelations among them.Different growth phase of Coolia monotis were simultaneously measured,and then growth curves were drew basis on the data from different methods.The results showed the best concordance between Microscope and Chla containing measurement methods,and then Visible spectrophotography,the worst was Fluorescence spectrophotography.Particularly,for Chla containing measurement method,the operating procedures are simple,accurate,suitable for wide cell-density ranges and batch measurements.Therefore,Chla containing measurement is convenient,high-efficiency and reliable approach for cell counting of Coolia monotis.

关 键 词：有毒甲藻 细胞计数法 生长曲线 

分 类 号：Q178[生物学—水生生物学]