超高压液相色谱-串联质谱测定水产养殖环境中的痕量氯霉素  

Determination of chloramphenicol in aquaculture using ultra pressure liquid chromatography coupled with tandem mass spectrometry

作　　者：张星炜[1] 房宝玲[2] 李君华[3] 

机构地区：[1]邯郸市环境保护局,河北邯郸056000 [2]邯郸学院,河北邯郸056000 [3]河北省科学院生物研究所,河北石家庄050081

出　　处：《河北省科学院学报》2012年第4期63-66,共4页Journal of The Hebei Academy of Sciences

摘　　要：本研究应用固相萃取及超高压液相色谱/质谱联用技术,建立了水产养殖环境中痕量氯霉素的分析方法。以空白的养殖渔塘水为基底,进行加标回收试验,加标质量浓度分别为0.1、1.0、5.0ng/mL时的回收率为81.0%～105.5%,相对标准偏差(RSD)在1.4%～9.5%之间(n=5),养殖渔塘水中的检出限为0.2ng/mL。结果,本研究所建立的方法成功对4个养殖渔塘进行了检测,检出了氯霉素的质量浓度在0.9～10.0ng/L之间,为以后的进一步分析研究奠定了基础。The study setup a analytical method for simultaneous determination of chloramphenicol in aquaculture using solid phase extraction-ultra pressure liquid chromatography coupled with tandem mass spectrometry.Using pond water as matrices,the recoveries and relative standard deviations(RSD,n=5) were 81.0%-105.5% and 1.4%-9.5% for chloramphenicol at 0.1ng/mL、1.0ng/mL and 5.0ng/mL spiking levels,respectively.The detection limits of the pond water were 0.2ng/mL.result:The method has been successfully applied to the survey of 4 aquaculture.The analytical method detected chlorammphenicol mass concentration between 0.9~10.0ng/L,and laid a foundation for further study.

关 键 词：氯霉素 超高压液相色谱—质谱 固相萃取 水产养殖环境 

分 类 号：O657[理学—分析化学]