下呼吸道表皮葡萄球菌感染诊断与痰液半定量培养细菌数量的关系  

Sepicermidis in lower respiratory tract infection diagnosis and bacteria quantitative relation in semi-quantitative cultured sputum

作　　者：李键[1] 杨肇立[1] 陈旭[1] 周文[1] 李俊如[1] 杨锦云[1] 任萍[1] 黄静[1] 

机构地区：[1]凉山彝族自治州第一人民医院检验科,四川西昌615000

出　　处：《四川医学》2012年第11期1877-1879,共3页Sichuan Medical Journal

基　　金：四川省卫生厅科研课题(编号:080160)

摘　　要：目的探索自然咳出痰标本筛检表皮葡萄球菌的指标。方法每份痰标本均同时涂片和接种。涂片观察标本质量,以白细胞吞噬菌或白细胞浓集区域优势菌为拟分离菌;培养采用半定量十二级法。查阅2005年8月1日～2011年8月31日住院患者痰内筛检出表皮葡萄球菌者病历,统计分析首次培养的细菌数量、标本质量与出院诊断的关系。结果表皮葡萄球菌101株,其首次培养的相对数量为0.1、≥0.2的临床诊断例数/总例数(阳性率)分别为2/10(20.00%)、54/91(59.34%);二者比较,=11.75,P<0.005。首次培养的标本质量为A/B级、C级的临床诊断例数/总例数(阳性率)分别为32/56(57.14%)、24/45(53.33%);二者确诊率比较,=0.55,0.75<P<0.90。结论按规定要求采集自然咳出痰标本,若其半定量12级法培养出涂片拟分离菌对应性表皮葡萄球菌的相对数量>该菌口咽部平均携带数量时,应作药敏试验并报告医生。Objective Explore how to screen S.epicermidis in the natural cough sputum.Methods Smear and inoculate each sputum sample at the same time.Observe two things.One is the quality of samples,the other is these bacteria phagocytized by white blood cells or the dominant bacteria in the white blood cells centralized region,which will be isolated.The inoculated sputum samples are cultured by 'semi-quantitative 12 order method'.Search the patients' medical records from August 1st,2005 to August 31st,2011,who are screened out S.epicermidis from their sputum.Then analyses the relation between the quantity of bacteria and diagnosis and the relation between quality of sample and diagnosis.Results There are 101 strains of S.epicermidis in the medical records.Among the cultured S.epicermidis,the positive rate of relative number of 0.1 is 20.00%,and the positive rate of relative number ≥ 0.2 is 59.34%.Diagnosis rate between the two,=11.75,Ρ<0.005.Besides,the positive rate of A/B grade sample is 54.72%,and the positive rate of C grade sample is 56.25%.Diagnosis rate between the two,=0.43,0.75<P<0.90.Conclusion From the approved natural cough sputum,if S.epicermidis,which is cultured by 'semi-quantitative 12 order method',is according to the smear,and its amount is more than the average carrying amount in pharyngo-oral cavity,do an antibiotic susceptibility test and report the rssult to the doctor.

关 键 词：表皮葡萄球菌 下呼吸道感染 痰半定量培养 细菌数量 

分 类 号：R446.5[医药卫生—诊断学]