人参皂苷Rg3、干扰素α联合与单一用药治疗血吸虫病肝纤维化疗效对比  

Comparison of Combined and Respective Effects of Ginsenoside Rg3 and Interferon-α on Schistosomiasis Japonica Caused Hepatic Fibrosis in Mice

作　　者：曾瑾[1] 李飞[1] 贾雪梅[1] 李翠英[1] 王红[1] 

机构地区：[1]昆明医科大学寄生虫学教研室,云南昆明650500

出　　处：《昆明医科大学学报》2012年第5期15-17,27,共4页Journal of Kunming Medical University

基　　金：云南省科技厅自然科学资基金助项目(2010ZC106)

摘　　要：目的比较人参皂苷Rg3、IFN-α联合用药与单一用药抗日本血吸虫病肝纤维化的疗效.方法将56只雄性IcR小鼠随机分为5组(A组为正常对照组,B、C、D、E组为治疗组),除A组8只外,其余B、C、D、E每组各12只.以腹部贴片法经皮肤感染B、C、D、E组小鼠,每只小鼠感染尾蚴18～22条.10周末,以每鼠吡喹酮1次性灌胃(300 mg/kg体重)杀虫治疗.继而分别以人参皂苷Rg3联合IFN-α使用和2者分别单独治疗B、C、D组小鼠,连续8周;第18周末,取各鼠肝组织分别作苏木素-伊红(HE)染色和苦味酸-酸性品红(VG)染色,观察分析各组小鼠肝脏胶原面积百分比及肝纤维化程度(SSS评分),以比较人参皂苷Rg3和IFN-α联合用药与单一用药的治疗效果.结果肝纤维化小鼠经人参皂苷Rg3、IFN-α联合或单一分别治疗8周后,肝脏仍有不同程度增大,暗褐色,质地略韧.肝脏内纤维增生、炎细胞浸润等病变比模型组减轻.3组治疗组肝脏胶原面积百分比均降低(P<0.05);SSS评分均低于实验对照组2分以上(P<0.05);3治疗组间胶原面积百分比及SSS评分没有统计学差异(P>0.05).结论吡喹酮治疗后,人参皂苷Rg3和IFN-α联合用药与单一用药一样,均具有改善小鼠日本血吸虫病肝纤维化作用,但2者合用疗效不优于单独用药,没有协同作用.Objective To compare the combined and respective effects of ginsenoside Rg3 and IFN-α on murine hepatic fibrosis due to Schistosoma japonicum.Methods 56 adult male IcR mice were randomized into 5 groups: A,B,C,D and E group.Each group had 12 mice except for group A with 8.Group A was a normal control group.Mice in group B,C,D and E were infected with 18～22 cercariae of S.japonicum in one day via abdominal skin puncture respectively.10 weeks later,the infected mice in the group B,C,D and E were treated with PZQ in a single oral dose to kill adult worms.Then,from the next day,mice in group B were treated with Ginsenoside Rg3 combined with IFN-α,mice in group C and D were treated with Ginsenoside Rg3 and IFN-α,respectively;mice in group E had no treatment,once a day for 8weeks.After 8-week treatment,liver tissue samples of the paraffin-embedded sections were made into serial 4 μm slices and were stained with hematoxylin eosin(HE)and van gieson(VG)to observe the histological changes,measure percentages of collagen fiber content in the liver tissue using graphic computing,and assess the scores through the semi-quantitative score scheme(SSS)or chronic hepatitis and liver fibrosis.Results After 8 weeks of treatment,mice in the B,C andD treatment groups had an enlarged liver.The liver was firm and dark brown in color,but the degree of fiber hyperplasia and inflammatory infiltration were mitigated compared to the group E.The mean percentages of the collagen content in the groupB,C and D,were lower than those of the group E(P<0.05).Meanwhile,the SSS scores of the group B,C and D were lower 2 scores at least than the group E,respectively(P<0.05).Conclusions Combined and respective ginsenoside Rg3 and IFN-α have similar anti-hepatofibrosis effects on schistosomiasis-induced hepatic fibrosis in mice after treatment of PZQ.However,in terms of the effects of anti-hepatofibrosis,there are no statistically significant differences among these three treatment groups.The effect of Rg3 combined with IFN-α on anti-hepatofibros

关 键 词：肝纤维化 血吸虫病 人参皂苷RG3 干扰素Α 

分 类 号：R383.2[医药卫生—医学寄生虫学]