大鼠海马齿状回、杏仁核突触外GABAA受体表达与生殖周期雌孕激素变化相关性研究  

Correlation between Expression of GABAA Extrasynaptic Receptors in Hippocampus Dentate Gyrus,Amygdala and Alterations of Estrogen and Progesterone during Rat Estrus Cycle

作　　者：张庭元[1] 郑小敏[1] 赵承军[2] 秦毅[2] 张晓光[1] 杨文娟[3] 马会明[1] 孙苗[1] 王燕蓉[1] 

机构地区：[1]宁夏医科大学生育力保持省部共建教育部重点实验室,宁夏生殖与遗传重点实验室,银川750004 [2]宁夏医科大学基础医学院人体解剖与组织胚胎学系,银川750004 [3]宁夏医科大学,银川750004

出　　处：《宁夏医科大学学报》2012年第8期764-767,封2,共5页Journal of Ningxia Medical University

基　　金：国家自然科学基金(30960114;31060140);宁夏医科大学特殊人才启动项目(XT201002)

摘　　要：目的明确生殖周期中卵巢甾体激素波动是否引发特定脑区神经元突触外GABAA受体表达变化及相关性。方法阴道涂片法筛选出具有完整生殖周期的大鼠,并将其分为两组,动情期组与动情间期组;Elisa检测每组大鼠血清雌孕激素含量;免疫组化法观察每组大鼠海马齿状回、杏仁核突触外GABAA受体δ亚基的表达;分析各组大鼠血清孕激素含量与突触外GABAA受体δ亚基表达的相关性。结果动情期组血清雌激素含量高于动情间期组,而孕酮含量则低于动情间期组,组间差异具有统计学意义(P<0.05)。海马齿状回、杏仁核GABAA受体δ亚基的免疫组化结果显示,阳性表达动情间期组高于动情期组,差异具有统计学意义(P<0.05);海马齿状回、杏仁核突触外δ亚基阳性结果 IOD值随着动情间期与动情期孕酮浓度值的增加而增加,两变量呈线性相关。结论动情间期高水平的孕激素可致大脑海马齿状回、杏仁核突触外GABAA受体高表达。Objective To definite the alteration of extrasynapse GABAA receptor expression followed by the ovarian steroid hormone.and the correlation with the fluctuation of the ovarian steroid hormone.Methods The rats with well-established ovarian cycles were used.Two stages of the estrous cycle were chosen for comparison: the estrus phase and the diestrus phase.The Elisa kit was used to detect the serum concentrations of estrogen and progesterone.Immunohistochemical method were used to marker the difference expression of subunitδin rats during estrus and diestrus.The relationship between the level of progesterone and extrasynaptic GABAA receptor subunit δ expression was analyzed.Results The levels of estrus serum estrogen were significantly higher than those of the diestrus group(P<0.05).The levels of estrus serum progesterone were significantly lower than those of the diestrus group(P<0.05).In the rat hippocampus dentate gyrus,amygdala,we found that the difference of the positive expression about subunit δ in both groups was significant(P<0.05).The IOD value of subunit δ-positive increase in hippocampus dentate gyrus,amygdala was parallel to the progesterone concentration increase during diestrus and estrus.By correlation analysis,we found a linear correlation between two variables in a certain range.Conclusion The high level of progesterone during diestrus can cause high expression of extrasynaptic GABAA receptor subunit δ in hippocampus dentate gyrus and amygdale.

关 键 词：海马 杏仁核 突触外GABAA受体δ亚基 生殖周期 大鼠 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]