人骨形态发生蛋白7在中国仓鼠卵巢细胞中的表达研究  

Expression of Human Bone Morphogenetic Protein 7 in Chinese Hamster Ovary Cells

作　　者：杨允[1,2] 邓春梅[1,2] 韩明[1,2] 黄艺娜[1] 黄义德[1,2] 

机构地区：[1]福建师范大学生命科学学院,福建福州350108 [2]福建省发育与神经生物学重点实验室,福建福州350108

出　　处：《生物技术》2012年第2期23-26,共4页Biotechnology

基　　金：教育部博士点基金项目(No.20103503120002)资助

摘　　要：目的:构建重组人骨形态发生蛋白-7(rhBMP7)表达质粒,并研究其在中国仓鼠卵巢细胞中的表达。方法:将hBMP7重组表达质粒电转到中国仓鼠卵巢细胞(CHO)中,并用DOT-BLOT和ELISA方法分析检测rhBMP7在重组CHO细胞中的表达。结果:hBMP7 cDNA整合到CHO细胞基因组中并被转录。点杂交和ELISA检测证实rhBMP7在CHO细胞中得到表达。结论:hBMP7成功在CHO表达系统中得到表达。Objective:Construction of the recombinant human bone morphogenetic protein 7(rhBMP7) expression vector and expression of rhBMP7 in Chinese Hamster Ovary(CHO) cells.Method:The expression vectors containing hBMP7 cDNA was transformed into CHO cells by electroporation and expression levels of rhBMP7 was analyzed by Dot blot and ELISA.Result:hBMP7 cDNA was integrated into genome of CHO cells and was transcribed in recombinant cells.Expression of rhBMP7 was con-firmed by Dot blot and ELISA.Conclusion:hBMP7 was successfully expressed in CHO expression system.

关 键 词：人骨形态发生蛋白- 中国仓鼠卵巢细胞 重组表达 

分 类 号：Q78[生物学—分子生物学]