HPLC法测定同仁大活络丸中黄芩苷的含量  

Determination of baicalin in Tongren Dahuoluo Pills by HPLC

作　　者：张海鸣[1] 刘子沐[1] 王成芳[1] 费超[1] 杜树山[1] 

机构地区：[1]北京师范大学资源学院教育部资源药物工程研究中心,北京100875

出　　处：《西北药学杂志》2012年第1期9-11,共3页Northwest Pharmaceutical Journal

基　　金：北京教育委员会共建项目建设计划(编号:SYS100270430)

摘　　要：目的建立同仁大活络丸中黄芩苷的含量测定方法。方法采用高效液相色谱法。色谱柱:C18(150mm×3.9mm,5μm);流动相:甲醇-1mL.L-1磷酸溶液(40∶60);检测波长:280nm;流速:1.0mL.min-1;柱温:30℃。结果黄芩苷在0.07～0.36μg范围内,峰面积与进样量呈良好的线性关系(r=0.999 8),黄芩苷的平均回收率为95.7%,RSD为0.5%。结论该方法简便、准确,可用于控制同仁大活络丸制剂的质量。Objective To establish an accurate method for the determination of baicalin in Tongren Dahuoluo Pills.Methods C18(150 mm×3.9 mm,5 μm) column in an oven at 30 ℃ was used with a mobile phase consisting of methanol phosphoric acid(40∶60).UV detection wavelength was 280 nm,and the flow rate was 1.0 mL·min-1.Results The linear ranges of baicalin was 0.07~0.36 μg(r =0.999 8).The average recovery was 95.7%(RSD=0.5%).Conclusion The method is simple,accurate,and can be used for the quality control of Tongren Dahuoluo Pills.

关 键 词：同仁大活络丸 黄芩苷 高效液相色谱法 含量测定 

分 类 号：R286[医药卫生—中药学]