艾灸足三里穴、关元穴对小鼠运动耐力及肾脏组织抗氧化损伤的影响  

Effects of applying moxibustion at Zusanli and Guanyuan acupoints on rats' exercise endurance and an ti-oxidant injury of kidney tissue

作　　者：华岩[1] 刘斌[2] 张可斌[1] 

机构地区：[1]钦州学院体育学院,广西钦州535000 [2]周口师范学院体育学院

出　　处：《中国康复医学杂志》2012年第11期1036-1040,共5页Chinese Journal of Rehabilitation Medicine

基　　金：广西教育厅科研立项项目(201106LX531);钦州学院科研项目(2012XJKY-15B)

摘　　要：目的:通过建立长期递增负荷训练、力竭训练游泳运动模型,探讨艾灸足三里穴、关元穴对长期训练小鼠运动耐力、血液生化指标及肾脏自由基代谢的影响。方法:实验小鼠30只,随机分为安静对照组(10只)、运动训练组(10只)、运动+艾灸组(10只)。测定运动训练组、运动+艾灸组小鼠力竭游泳时间,各组小鼠血红蛋白(Hb)、全血乳酸(Bla)、血清肌酸激酶(CK)、血清尿素氮(BUN)、肾脏组织丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)。结果:运动+艾灸组力竭运动时间显著延长;运动训练组力竭时与安静对照组相比,血浆Hb显著降低(P<0.05),Bla显著升高(P<0.05),血清CK、BUN显著升高(P<0.05),肾脏组织MDA含量显著升高(P<0.05),GSH-Px活性、SOD活性均显著降低(P<0.05);运动训练组力竭时与运动+艾灸组力竭时相比,血浆Hb显著升高(P<0.05),Bla显著降低(P<0.05),血清CK、BUN显著降低(P<0.05),肾脏组织MDA含量显著降低(P<0.05),GSH-Px活性、SOD活性均显著升高(P<0.05);运动+艾灸组力竭时与安静对照组相比,血浆Hb含量略高,但无显著差异(P>0.05),血浆Bla显著升高(P<0.05),血清CK、BUN显著升高(P<0.05),肾脏组织MDA含量略高,GSH-Px活性略低,但差异无显著性(P>0.05),SOD活性显著降低(P<0.05)。结论:艾灸足三里、关元穴可提高了机体的造血功能和循环系统功能,促进了机体Hb的再生与合成,减少体内Bla堆积,抑制肌细胞中CK溢出,降低血清中BUN含量,明显加快肾脏组织中自由基的清除,从而提高机体抗疲劳能力,增强机体的运动能力。Objective:To discuss the effects of applying moxibustion at zusanl(iST36)and guanyuan(RN4)acupoints on exercise endurance,blood biochemical indices,and free radicals metablolism of kidney tissue on the basis of establishing long-term incremental treadmill exercise and exhaustive swimming trainings rats models.Method:A total of 30 rats in this experiment were randomly divided into sedentary control group(n=10),training group(n=10),training plus moxibustion group(n=10).And then the exhaustive swimming time were measured in training group and training plus moxibustion group,to obtain those indices of hemoglobin(Hb),blood lactale(Bla),serum creatine kinase(CK),serum blood urea nitrogen(BUN),kidney malondialdehyde(MDA),glutathioc peroxidase(GSH-Px) and superoxide dismutase(SOD).Result:In training plus moxibustion group the exhaustive swimming time extended obviously;compared with sedentary control group in normal training group,plasma Hb decreased significantly(P<0.05),plasma Bla,serum CK,serum BUN,MDA increased significantly(P<0.05),activities of GSH-Px and SOD decreased significantly(P<0.05);compared with training group in training plus moxibustion group,plasma Hb increased obviously(P<0.05),plasma Bla,serum CK,serum BUN decreased obviously(P<0.05),the content of MDA decreased significantly(P<0.05),the activities of GSH-Px and SOD increased significantly(P<0.05);compared with sedentary control group in training plus moxibustion group,the plasma Hb increased relatively,but without significant difference(P>0.05),plasma Bla increased significantly(P<0.05),serum CK,serum BUN increased significantly(P<0.05),the content of MDA increased relatively and GSH-Px was lower,but without significant difference(P<0.05),activity of SOD increased significantly(P<0.05).Conclusion:Moxibustion at zusanli(ST36) and guanyuan(RN4) acupoints can enhance organism hematopoietic function and circulatory system function,promote the regeneration and synthesis of Hb in organism,reduce Bla accumulation,inhibit CK overflowed in muscle cells,decrea

关 键 词：艾灸 足三里穴 关元穴 运动耐力 肾脏 自由基 

分 类 号：G804.2[文化科学—运动人体科学]