HPLC同时测定麦冬中3种高异黄酮含量  

Simultaneous determination of three homoisoflavonoids in Ophiopogon japonicus by HPLC

作　　者：曾品涛[1] 周慧[1] 郑一敏[1] 胥秀英[1] 傅善权[1] 

机构地区：[1]重庆理工大学药学与生物工程学院,重庆400054

出　　处：《中国中药杂志》2012年第1期71-74,共4页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创制"科技重大专项(2010ZX09401-306-2-23);重庆市科委产业化项目(CSTC;2008AB5116)

摘　　要：采用反相高效液相色谱法同时测定麦冬中6-甲酰基沿阶草酮甲、甲基沿阶草酮甲和沿阶草酮甲的含量,选用乙腈-0.5%磷酸溶液(58∶42)作为流动相,检测波长为296 nm(0～14 min),275 nm(14～22 min)。6-甲酰基沿阶草酮甲、甲基沿阶草酮甲和沿阶草酮甲检测的线性范围分别为0.165～0.990(r=0.999 9),0.153～0.918(r=0.999 9),0.270～1.620μg(r=0.999 9),平均加样回收率分别为99.76%(RSD 0.95%),99.86%(RSD 0.82%),99.41%(RSD1.1%)。研究所建立的反相高效液相色谱法法简便快速、准确度高,可用于麦冬药材的质量控制。Three homoisoflavonoids,6-aldehydo-3-ophiopogonanone A,methyl ophiopogonanone A and ophiopogonanone A from Ophiopogon japonicus were analyzed simultaneously by HPLC with acetonitrile-water containing 0.5% H3PO4(58∶42) as the mobile phase,and the detection wavelength was set at 296 nm(0-14 min) and 275 nm(14-22 min).The mean recoveries of three homoisoflavonoids were 99.41%-99.86%(RSD 0.82%-1.05%).The linear response ranges of.6-aldehydo-3-ophiopogonanone A,methyl ophiopogonanone A and ophiopogonanone A were 0.165-0.990 μg(r=0.999 9),0.153-0.918 μg(r=0.999 9),and 0.270-1.620 μg(r=0.999 9),respectively.This method was certified to be accurate and reliable and can be used for quality control of O.japonicus.

关 键 词：麦冬 高效液相色谱 -甲酰基沿阶草酮甲 甲基沿阶草酮甲 沿阶草酮甲 含量测定 

分 类 号：S567.232[农业科学—中草药栽培]