“一测多评”法测定三黄片中的大黄蒽醌类成分  

Determination of four anthraquinones in Sanhuang tablets by QAMS

作　　者：王钰莹[1,2,3] 冯伟红[2,3] 杨菲[2,3,4] 张启伟[2,3] 吉丽娜[2,3,4] 李东影[2,3,4] 王智民[2,3] 

机构地区：[1]首都医科大学中医药学院,北京100069 [2]中国中医科学院中药研究所,北京100700 [3]中药质量控制技术国家工程实验室,北京100700 [4]天津中医药大学,天津300193

出　　处：《中国中药杂志》2012年第2期212-217,共6页China Journal of Chinese Materia Medica

基　　金：中医药行业科技专业项目(200707009);国家“重大新药创制”科技重大专项(2009ZX09301-005,2009ZX09308-003)

摘　　要：目的:建立"一测多评"法测定三黄片中大黄蒽醌类成分含量的分析方法,并进行方法学考察。方法:以三黄片为研究对象,建立大黄酸(rhein)、大黄酚(chrysophanol)和大黄素甲醚(physcion)与内参物大黄素(emodin)间的相对校正因子。分别采用外标法和"一测多评"法测定三黄片中4种蒽醌类成分的含量,并将"一测多评"法的计算值与外标法实测值用相对误差进行评价,以验证"一测多评"法的合理性、可行性和可重复性。结果:三黄片中蒽醌类成分间的相对校正因子分别为f 254nm大黄酸/大黄素=1.13,f 254nm大黄酚/大黄素=1.46,f 254nm大黄素甲醚/大黄素=1.01。"一测多评"法的计算结果与外标法的实测值之间没有显著性差异,实验所得的相对校正因子可信。结论:"一测多评"法可作为一个新的质量评价模式用于三黄片中蒽醌类成分的质量评价。Objective:To establish a quality evaluation method,quantitative analysis of multi-component with a single-marker(QAMS) to determine the contents of four rhubarb anthraquinones in Sanhuang tablets.Method:Emodin was used as the internal reference substance,the relative correlation factors(RCF) of rhein,chrysophanol and physcion to emodin were calculated and evaluated.The contents of these four anthraquinones were determined by the external standard method and QAMS,respectively.Rationality,feasibility and repeatability of the QAMS method was verified by comparing the results obtained from the two different methods.Result:RCFs of rhein,chrysophanol and physcion to emodin against Sanhuang tablets were 1.13,1.46 and 1.01,respectively,indicating good reproducibilities.The two methods did not show significant difference in results.Conclusion:The QAMS method can be used a new quality assessment model for quantity of anthraquinones in Sanhuang tablets.

关 键 词：一测多评 HPLC 三黄片 蒽醌 相对校正因子 

分 类 号：R286[医药卫生—中药学]