HPLC同时测定乌灵胶囊中多组分的含量  

Determination of multiple constituents in Wuling capsules by HPLC simultaneously

作　　者：陈勇[1] 陆静娴[1] 祝明[1] 罗镭[1] 

机构地区：[1]浙江省食品药品检验所,浙江杭州310004

出　　处：《中国中药杂志》2012年第2期218-221,共4页China Journal of Chinese Materia Medica

基　　金：国家"十一五"科技支撑项目(009ZX09308-004)

摘　　要：目的:建立同时测定乌灵胶囊中5-hydroxymellein,5-carboxylmellein,金雀异黄素及5-甲基蜂蜜曲霉素含量的方法。方法:采用高效液相色谱法,Kromasil KR100-5C18色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-0.2%磷酸溶液,梯度洗脱;流速1.0 mL·min-1;柱温30℃。结果:5-hydroxymellein在7.86～157.2 ng线性关系良好,平均回收率为101%,RSD1.3%;5-carboxylmellein在9.57～191.4 ng线性关系良好,平均回收率为98.6%,RSD 1.5%;金雀异黄素在28.80～576.0 ng线性关系良好,平均回收率为98.6%,RSD 1.8%;5-甲基蜂蜜曲霉素在21.46～429.2 ng线性关系良好,平均回收率为99.2%,RSD 1.8%。结论:该研究建立的方法可行,重复性好,可用于乌灵胶囊的质量控制。Objective:To establish a method for the content determination of 5-methylmellein,5-hydroxymellein,5-carboxylmellein and genistein in Wuling capsules simultaneously by HPLC.Method:Four components were determined by HPLC on a Kromasil KR100-5C18 column(4.6 mm×250 mm,5 μm) with acetonitrile and 0.2% phosphoric acid as mobile phase in a gradient elution.The flow rate was 1.0 mL·min-1,and the column temperature was set at 30 ℃.Result:5-hydroxymellein showed a good linear relationship at the range of 7.86-157.2 ng,the average recovery was 101.0% with RSD 1.3%.5-carboxylmellein showed a good linear relationship at the range of 9.57-191.4 ng,the average recovery was 98.6% with RSD 1.5%.Genistein showed a good linear relationship at the range of 28.80-576.0 ng,the average recovery was 98.6% with RSD 1.8%.5-methylmellein showed a good linear relationship at the range of 21.46-429.2 ng,the average recovery was 99.2% with RSD 1.8%.Conclusion:The established method is feasible and the repeatability is good.The method can be used for quality control of Wuling capsules.

关 键 词：乌灵胶囊 5-hydroxymellein 5-carboxylmellein 金雀异黄素 5-甲基蜂蜜曲霉素 

分 类 号：R286.0[医药卫生—中药学]