紫外-可见分光光度法测定川明参茎叶中总黄酮含量  

Determination of total flavonoids in stemleaves of Chuanminshen violaceum by UV-Vis spectrophotometry

作　　者：明凯利[1] 宋芳芳[1] 陈朝霞[1] 张梅[1] 

机构地区：[1]成都中医药大学药学院中药材标准化教育部重点实验室中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都610075

出　　处：《中药与临床》2012年第5期3-4,8,共3页Pharmacy and Clinics of Chinese Materia Medica

基　　金：四川省科技厅科技富民强县专项行动计划项目

摘　　要：目的:建立川明参茎叶中总黄酮含量测定方法。方法:采用回流提取法提取川明参茎叶中的总黄酮,以芦丁为对照品,采用紫外-可见分光光度法于506nm处测定。结果:在0.0212～0.0636mg/mL范围内,对照品芦丁的吸光度与浓度线性关系良好(r=0.9992)。平均加样回收率为97.03﹪,RSD=2.3﹪(n=6)。结论:该方法准确、简便,可用于川明参茎叶中总黄酮的含量测定,为川明参进一步综合开发利用奠定了一定的实验基础。Objective:To establish the determination method of the total flavonoids in stemleaves of Chuanminshen violaceum.Method:Reflux extracting method was used to extract the total flavonoids.The contents of total flavonoids were determined by UV-Vis spectrophotometry at wavelength of 506 nm with rutin as standard substance.Result:The linear range of rutin were 0.0212 mg﹒mL-1～0.0636 mg﹒mL-1(r=0.9992) with average recovery of 97.03%(RSD=2.3%,n=6).Conclusion:The method is accurate and simple,and it can be used for the content determination of total flavonoids in stemleaves of Chuanminshen violaceum.The research has laid a foundation for the utilization of Chuanminshen violaceum.

关 键 词：川明参 总黄酮 紫外-可见分光光度法 

分 类 号：R284[医药卫生—中药学]