油茶胚状体发生诱导初步研究  

A Preliminary Study on the Occur and Induction of Embryoid of Camellia oleifera

作　　者：黄莉雅[1] 陈国臣[1] 马锦林[1] 

机构地区：[1]广西林业科学研究院,广西南宁530002

出　　处：《福建林业科技》2012年第4期97-100,116,共5页Journal of Fujian Forestry Science and Technology

基　　金：广西区科技厅农转资金项目"油茶高产无性系扩繁示范"(桂科转10100017-1)

摘　　要：为探求适宜油茶胚状体发生诱导培养条件,以油茶去壳成熟种子为外植体,进行胚状体发生诱导试验。结果表明:在其他培养条件相同的情况下,不同的油茶品种、不同的植物生长调节剂配比对诱导油茶胚状体发生的影响各异:2,4-D 2.0mg.L-1+KT 1.0 mg.L-1对胚性愈伤组织的诱导率最高,达到73%;6-BA 0.6 mg.L-1+NAA 2.0 mg.L-1对胚状体的诱导率最高,达到73%,诱导效果最佳。In order to find the suitable culture conditions of embryoids induction of Camellia oleifera,the mature seeds of the Camellia oleifera were shelled as explants to test somatic embryogenesis induction.The results showed that under the same other conditions,different varieties of Camellia oleifera,different ratios of plant hormones had different impacts on embryoids induction for Camellia oleifera.When the concentration of 2,4-D and KT were 2.0 mg·L-1 and 1.0 mg·L-1,the embryogenic callus induction rate was the highest at 73% and the induction effect was the best.

关 键 词：油茶 胚状体 诱导 

分 类 号：S794.4[农业科学—林木遗传育种]