一株源于煤矸石的T.f菌分离及其生理特性  

Isolation of Thiobacillus Ferrooxidans from Coal Waste and Its Living Characteristics

作　　者：岳兴玲[1] 王海霞[2] 

机构地区：[1]山东省环境保护科学研究设计院,山东济南250013 [2]济南大学资源与环境学院,山东济南250022

出　　处：《济南大学学报（自然科学版）》2013年第3期254-257,共4页Journal of University of Jinan(Science and Technology)

基　　金：山东省自然科学基金(ZR2010DQ005);济南大学科研基金(XKY0933)

摘　　要：从煤矸石中分离获得一株氧化亚铁硫杆菌(T.f菌),测定培养时间、温度、pH值和接种量对菌株的生长代谢及氧化二价铁的影响,探讨菌株的生理生化特性。结果表明,该菌株是革兰氏阴性菌,可以利用亚铁、单质硫和葡萄糖生长,且在亚铁葡萄糖混合培养基中也能生长,但不能利用蛋白胨生长;该菌株为嗜酸菌,其最适pH值为2.0～2.5,最适温度为30℃,Fe2+的氧化率最高达93%。Thiobacillus ferrooxidans(T.f) was isolated from coal wastes,and its physiological characteristics and environmental factors for growth,such as cultivation time,temperature,pH value and inoculation amount,were studied.The results show that this strain of T.f is gram-negative,and can grow with Fe2+,pure sulfur and ferrous glucose mixed media,but can not grow with protein peptones.As a kind of acidophilus,T.f is suitable to grow in the environment with pH 2.0~2.5,temperature 30 ℃,and up to 93% oxidation ratio of Fe2+.

关 键 词：氧化亚铁硫杆菌 煤矸石 菌株分离 生理特性 

分 类 号：Q93[生物学—微生物学]