NO及NOS在老年Ⅰ期压疮大鼠骨骼肌组织细胞凋亡中的作用  

Effect of NO/NOS on apoptosis of skeletal muscle tissue cells in elderly rats with pressure sore in phase Ⅰ

作　　者：王艳[1] 周守凤[1] 高金华[1] 陈慧敏[1] 郑国荣[1] 刁波[2] 

机构地区：[1]中国人民解放军广州军区武汉总医院消化内科,湖北省武汉市430070 [2]中国人民解放军广州军区武汉总医院神经外科医学实验中心,湖北省武汉市430070

出　　处：《实用老年医学》2013年第7期598-601,共4页Practical Geriatrics

基　　金：湖北省自然科学基金资助项目(2012FFB06806);湖北省卫生厅2013-2014年度科研项目(HL2012-8)

摘　　要：目的观察一氧化氮(NO)及一氧化氮合酶(NOS)在老年Ⅰ期压疮大鼠骨骼肌组织细胞凋亡中的作用,进一步探索老年压疮发生机制。方法将老年SD大鼠12只随机分成对照组和模型组,各6只。对照组不受压、模型组承受22.47kPa压强2h后2组大鼠一起实施安乐死。实验终点,光镜观察皮肤肌肉组织病理学变化;检测肌组织中NO、NOS和超氧化物歧化酶(SOD)的活性和含量;检测皮肤和肌肉组织中细胞凋亡指数(AI)的变化。结果对照组镜下观察皮肤完整,颜色、结构正常;模型组大鼠复层鳞状上皮较薄,结构层次欠清晰。肌纤维空泡变、轻度水肿,有蜡样变性。肌组织萎缩、有自溶现象。2组SOD、NO、NOS、AI比较,差异有统计学意义(均P<0.01)。NO、NOS与AI呈明显的正相关。结论 NO、NOS在老年Ⅰ期压疮大鼠骨骼肌组织细胞凋亡过程中发挥介导作用。NO导致的脂质过氧化反应与细胞凋亡可能是启动早期压疮发生的分子调控机制之一。Objective To observe the effect of NO and NOS during the process of cell apoptosis in elderly rats with pressure sore (phase Ⅰ), and to further investigate the mechanism of pressure sore. Methods A total of 12 rats were divided into model group (6 rats) and control group (6 rats). A pressure of 22.47kPa was pushed on model group while no pressure on control group, and two groups of rats were put to death with euthanasia after 2 h. Finally, the pathological changes of the skin and muscle were observed under light microscope, and the levels and activity of NO, NOS and SOD, and the apoptosis index (AI) were detected. Results In model group, the stratified squamous epithelium cells showed thin, and the structure became unclear, vacuolar alteration in muscle fibers, mild edema, waxy degeneration, muscle tissue atrophy and autolysis, while the color and structure of skin and muscle were normal in control group. There were signi-ficant differences between the two groups in the levels and activity of NO, NOS and SOD. NO, NOS were positively correlated with the process of cell AI. Conclusions NO and NOS play a key mediated role in the process of skin and muscle cell apoptosis in elderly rat with pressure sore (phase Ⅰ). The lipid peroxidation and apoptosis induced by NO may be one of the potential mechanisms in the formation of early pressure sore.

关 键 词：一氧化氮 一氧化氮合酶 压疮 细胞凋亡 动物实验 

分 类 号：R632.1[医药卫生—外科学]