辛伐他汀通过氧化应激抑制人气道平滑肌细胞增殖  

Simvastatin inhibits human airway smooth muscle cells proliferation by oxidative stress

作　　者：沈奕[1] 查王健[1] 钱艳[1] 李娟[1] 黄茂[1] 

机构地区：[1]南京医科大学第一附属医院呼吸科,210029

出　　处：《中华哮喘杂志（电子版）》2013年第2期84-88,共5页Chinese Journal of Asthma(Electronic Version)

基　　金：科技部科技重大专项"重大新药创制"(2011zx09302-003-02);江苏高校优势学科建设工程资助项目

摘　　要：目的探讨辛伐他汀(sim vastatin,Sim)对血小板活化因子(platelet activating factor,PAF)诱导的人气道平滑肌细胞(human airway smooth muscle cells,HASMCs)中活性氧(reactive oxidant stress,ROS)的生成及对细胞增殖的影响。方法用CCK-8法检测细胞增殖,2′,7′-二氯荧光乙酰乙酸(DCFH-DA)染色,用荧光显微镜成像观察细胞荧光强弱,流式细胞术检测细胞内ROS水平。结果 PAF(10-6mol/L,24h)的增殖率是对照组的(1.77±0.51)倍,Sim(10-5mol/L)(0.67±0.18)可抑制PAF促增殖作用(P<0.01)。PAF10-6mol/L组平均荧光强度(mean fluorescence intensity,MFI)(98.89±1.28)较阴性对照组(76.79±6.05)明显增强,PAF10-6mol/L+Sim10-5mol/L组(66.40±2.87)较PAF10-6组减弱(P<0.01)。结论 Sim可抑制PAF诱导的HASMCs增殖,可能是通过减少ROS的生成起作用。Objective To investigate whether simvastatin may influence human airway smooth muscle cell proliferation induced by platelet activating factor through ROS formation.Methods Cell proliferation was analyzed according to the protocol of CCK-8assay.Fluorescence microscope was used to observe fluorescence intensity of each group.Intracellular reactive oxygen species was measured by the flow cytometer using an oxidationsensitive fluorescent probe , 2′7′-dichlorofluorescin diacetate ( DCFH-DA ) .Results Our data demonstrated that PAF induced HASMCs proliferation with the maximal effect seen at 10-6 M and 24h , the proliferation rate was ( 1.77±0.51 ) as fold as the control group.Simvastatin ( at the concentration of 10-5 M ) was ( 0.67±0.18 ), inhibited HASMCs proliferation induced by PAF ( P <0.01 ) .Compared with the negative control group ( 76.79±6.05 ), PAF-treated group ( 98.89±1.28 ) led to a significant increase in DCF fluorescence , PAF combined with simvastatin group ( 66.40±2.87 ) decreased ROS level ( P <0.01 ) .Conclusions Simvastatin inhibited HASMCs proliferation induced by PAF partly through ROS formation.

关 键 词：人气道平滑肌细胞 辛伐他汀 血小板活化因子 活性氧 

分 类 号：R96[医药卫生—药理学] R58[医药卫生—药学]