刺槐素通过抑制NF-κB通路活化减少TNF-α诱导气道平滑肌细胞表达、分泌MCP-1  

Acacetin down-regulates TNF-α induced MCP-1 expression via blockade of NF-κB signaling pathway in human airway smooth muscle cells

作　　者：钱艳[1] 查王建[1] 沈奕[1] 李娟[1] 黄茂[1] 

机构地区：[1]南京医科大学第一附属医院呼吸内科,210029

出　　处：《中华哮喘杂志（电子版）》2013年第3期6-9,共4页Chinese Journal of Asthma(Electronic Version)

基　　金：科技部科技重大专项"重大新药创制";基金编号:2011zx09302-003-02;江苏高校优势学科建设工程资助项目

摘　　要：目的探讨刺槐素(Acacetin)对肿瘤坏死因子-α(TNF-α)诱导的人气道平滑肌细胞(HASMCs)分泌、表达单核细胞趋化蛋白-1(monocyte chemotactic protein1,MCP-1)的影响及可能的机制。方法 CCK-8法测HASMCs增殖活性;酶联免疫吸附法(ELISA)测HASMCs上清液中MCP-1含量;实时荧光定量PCR(RT-PCR)检测MCP-1mRNA表达量;蛋白印迹法(Western blot)检测HASMCs中磷酸化P65(P-P65)蛋白的表达情况。结果①Acacetin及NF-κB抑制剂JSH-23均能显著降低TNF-α诱导的HASMCsMCP-1生成,且Acacetin的抑制作用具有浓度依赖性(P<0.05);②同时,Acacetin及JSH-23均能显著减少TNF-α诱导的MCP-1mRNA的表达(P<0.05);③Acacetin能显著降低TNF-α引起的HASMCsP65蛋白磷酸化(P<0.05)。结论降低NF-κB通路的活化水平可能是Acacetin抑制TNF-α诱导HASMCs合成、分泌MCP-1及其mRNA,从而发挥抗炎作用的机制之一。Objective To investigate whether Acacetin can regulate TNF-α induced monocyte chemotactic protein1 (MCP-1) expression on human smooth muscle cells and its mechanism involved. Methods CCK-8 assay measured HASMCs proliferation; ELISA detected the levels of MCP-1 in the supernatants of HASMCs; Reverse transcriptase polymerase chain reaction (RT-PCR) detected the expression of MCP-1 mRNA in HASMCs; Western Blotting detectedP65 phosphorylation of HASMCs after TNF-α and/or Acacetin treatment.Results Our data showed that Acacetin and NF-κB activation inhibitor (JSH-23) could signiflcantly suppress TNF-α-induced MCP-1 protein and mRNA expression; Acacetin markedly suppressed TNF-α-induced P65 phosphorylation.Conclusions These results support that Acacetin exerts its anti-inflammatory activity by suppressing TNF-α-induced expression of MCP-1 via blockage of activation of NF-κB.

关 键 词：刺槐素 人气道平滑肌细胞 单核细胞趋化蛋白-1 肿瘤坏死因子-α NF-ΚB通路 气道炎症 

分 类 号：R28[医药卫生—中药学] R39[医药卫生—中医学]