生物合成埃博霉素的主要菌种研究  

The Study of Main Strains of Epothilone Bio-synthetic

作　　者：王超[1] 刘新利[1] 

机构地区：[1]山东轻工业学院山东省微生物工程重点实验室,山东济南250353

出　　处：《山东轻工业学院学报（自然科学版）》2013年第2期29-33,共5页Journal of Shandong Polytechnic University

基　　金：山东省科技发展计划(2012GSF12107)

摘　　要：目前国际上主要报道过三株埃博霉素原始产生菌:纤维堆囊菌So ce90、SMP44和So0157-2。关于埃博霉素的生物发酵研究也多集中于此三种菌及其诱变菌株。国内外也有许多单位致力于将埃博霉素合成酶基因簇进行异源表达。但由于埃博霉素对异源宿主菌具有细胞毒性,导致异源表达较为困难或产物产量不尽如人意,因此,目前大部分研究仍致力于对纤维堆囊菌菌种及其发酵条件的改良,以提高发酵产量。Sorangium cellulosum So ce90,SMP44,and So0157-2 were the most reported three international epothilone producing strains .Researches about the bio-fermentation of epothilone were also concentrated on these three strains .Epothilone synthase gene clusters heterologous expression was studied .However, epothilone was cytotoxic to the heterologous host , which makes it difficult of heterologous expression . Therefore,most of the research is still committed to the optimization of strains and their fermentation conditions to improve fermentation yield .

关 键 词：埃博霉素 纤维堆囊菌 生物合成 

分 类 号：TQ465[化学工程—制药化工]