急诊科护理人员手部细菌培养监测分析  

Bacteriological surveillance for hand hygiene of nursing staffs in department of emergency

作　　者：梅申聪 丁亚君 唐芳 朱长太 

机构地区：[1]苏州大学附属常州肿瘤医院,江苏常州市213001

出　　处：《热带病与寄生虫学》2012年第2期77-79,共3页Journal of Tropical Diseases and Parasitology

基　　金：国家重点基础研究发展计划子课题资助(2012CB518700)

摘　　要：目的调查急诊科护理人员手部细菌携带情况及细菌种类,监测其洗手效果,评估其执行手卫生的意义。方法对急诊科共20名护理人员进行手部细菌培养检测,分别在洗手前及洗手后进行采样。细菌鉴定及药敏主要由VITEK仪器完成。结果洗手前手部中位菌落数为2 CFU/cm^2,四分位距(interquartile range,IQR)为1-3.75 CFU/cm^2。洗手后中位菌落数为0CFU/cm^2,IQR为0-0 CFU/cm^2。同洗手前比较,洗手后菌落计数减少有统计学意义(P<0.05)。细菌鉴定及药敏结果显示:菌种分布较为广泛,以葡萄球菌属及肠杆菌科等条件致病菌为主;检出多重耐药菌3株。结论急诊科护理人员操作中手部存在一定程度细菌污染,而正确洗手及提高护理人员手卫生依从性,可有效清除细菌,有利于预防医院感染的发生。Objective To investigate the situation of bacteria carrying and classification on the hands of nursing staffs in the department of emergency,monitor the effect of hand-washing,and evaluate the significance of hand hygiene.Methods 20 nurses in the department of emergency were selected,and the samplings for the hands were conducted and cultivated in operation(before hand-washing) and after hand-washing,respectively. Bacterial identification and antimicrobial susceptibility testing were performed mainly by the VITEK instrument.Results The median colony number before hand-washing was 2 CFU(colony forming unit)/cm^2 and the interquartile range(IQR) was 1-3.75 CFU/cm^2.While after hand-washing,the median and IQR colony numbers were 0 CFU/cm^2 and 0-0 CFU/cm^2,respectively.There was a statistically significant reduction before hand-washing in colony count compared to that after hand-washing(P<0.05).The identification and susceptibility results showed that the distribution of bacteria species were widely,but mainly belonged to Staphylococcus and Enterobacteriaceae opportunistic pathogen.Three multidrug—resistant strains were found. Conclusion Bacterial contamination may exist on the hands of nurses in operation in the department of emergency, and proper hand-washing and higher compliance on hand hygiene for nurses can remove bacteria and contribute to the prevention of nosocomial infection effectively.

关 键 词：护理 手卫生 医院感染 细菌 

分 类 号：R4[医药卫生—临床医学] R39