叠鞘石斛与药典品种石斛酚含量对比研究  

A comparison of the gigantol content between Dendrobium aurantiacum Rchb.f. var. denneanum(Kerr.)Z.H.Tsi and pharmacopeia species

作　　者：陈佳江[1] 郭力[1] 许莉[1] 石媛慧[1] 郝普彦[1] 张廷模[1] 韩瑜 

机构地区：[1]成都中医药大学药学院 中药材标准化教育部重点实验室 中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都610075 [2]四川万安石斛产业开发有限公司,四川成都610213

出　　处：《中药与临床》2013年第1期9-10,18,共3页Pharmacy and Clinics of Chinese Materia Medica

基　　金：科技部"十二五"支撑项目-石斛规范化种植基地及其SOP优化;升级研究(NO.2011BAI13B02-8)

摘　　要：目的:建立叠鞘石斛与药典收载品种金钗石斛,鼓槌石斛中石斛酚的高效液相色谱含量测定方法.方法:Diamonsil C18(150 mm×4.6 mm,5μm)色谱柱,乙腈-0.5％冰醋酸(35:65)为流动相,检测波长280 nm,流速1 mg· mL-1,柱温30℃.结果:石斛酚在0.01228～0.2456 mg·mL-1 (r=0.9999)范围内与峰面积线性关系良好,平均回收率为100.39％,RSD为2.17％.结论:本方法操作简单、快捷、重现性好、准确率高,可用于上述三种石斛中石斛酚的含量测定.Objective: To develop a HPLC method for quantitative determination of gigantol in D.aurantiacum Rchb.f. var. denneanum (Kerr) Z.H.Tsi and species in Chinese Pharmacopoeia. Method: The determination was carried out on a Diamonsil Cl8(150 mm×4.6 mm,5 μm) at 30 ℃ with acetonitrile-0.5% acetic acid(35:65) as mobile phase at a flow rate of 1.0 mg﹒mL-1. And the detection wavelength was set at 270 nm. Result: The peak areas and injection amounts of gigantol had a good linear relationship in the range of 0.01228~0.2456 mg﹒mL-1(r=0.9999). The average recovery was 100.39%, and the RSD was 2.17%. Conclusion: The method is quick,simple and repeatable for simultaneous determination of gigantol in D.aurantiacum Rchb.f. var. denneanum(Kerr.)Z.H.Tsi, D.nobile Lindl and D.chrysotoxum Lindl.

关 键 词：叠鞘石斛 药典品种 石斛酚 高效液相色谱 含量测定 

分 类 号：R282.5[医药卫生—中药学]